Compound distribution in microfluidic devices

Patent metadata

Field	Value
Publication number	US-12019083-B2
Application number	US-202217834165-A
Country	US
Kind code	B2
Filing date	Jun 7, 2022
Priority date	Jun 27, 2019
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

First claim

The invention claimed is:

1. A method of determining compound distribution in a system, comprising:
a) providing a system and an experimental protocol for said system comprising one or more biological elements; wherein said one or more biological elements are contacted by a compound;
b) modifying said experimental protocol by excluding at least one of said one or more biological elements;
c) performing said modified experimental protocol; and
d) determining the distribution of said compound in said system using by measuring the concentration of said compound in said system.

2. The method of claim 1 , wherein said experimental protocol comprises introducing fluid flow into said system.

3. The method of claim 2 , wherein said experimental protocol comprises collecting effluent.

4. The method of claim 2 , wherein said experimental protocol comprises assaying said effluent.

5. The method of claim 1 , wherein said biological elements comprise cells.

6. The method of claim 1 , wherein said biological elements comprise biological coatings.

7. The method of claim 1 , wherein said system comprises one or more microfluidic devices.

8. The method of claim 1 , wherein said distribution of said compound is used to calculate error bars for results from said experimental protocol.

9. The method of claim 1 , wherein said distribution of said compound is used to calculate half maximal inhibitory concentration (IC50) for said experimental protocol.

Assignees

• Emulate Inc

Inventors

• Sliz Josiah
• Levner Daniel
• Zuckerman Brian
• Wen Norman
• Rubins Jonathan
• Shroff Tanvi
• Hinojosa Christopher David
• Ahn Grace
• Antontsev Victor
• Puerta Jefferson
• Conegliano David
• Kerns S Jordan

Classifications

• G01N2035/00544

  using fluid flow · CPC title

• G01N15/06

  Investigating concentration of particle suspensions (by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10) · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/0681

  Filter · CPC title

• G01N2035/0097

  monitoring reactions as a function of time · CPC title