Methods and devices for separation of blood components

Patent metadata

Field	Value
Publication number	US-12298296-B2
Application number	US-202217929203-A
Country	US
Kind code	B2
Filing date	Sep 1, 2022
Priority date	Nov 28, 2017
Publication date	May 13, 2025
Grant date	May 13, 2025

Abstract

The present disclosure relates to methods and devices for the separation of blood components including separation by rapid sedimentation, including in an automated fashion.

First claim

What is claimed is:

1. An automated method to separate previously fractionated blood components, the method comprising:
providing at least one fractionated blood sample from a sample vessel to a device comprising:
a piercing post to receive a sample, wherein the sample vessel and the piercing post are in fluid communication, and,
at least a first and second arm, each extending perpendicular to length of the piercing post/sample vessel complex in opposite directions, wherein each at least first and second arm has a bend at the distal end of the perpendicular segment, and a second portion of each arm which extends from the bend in a direction perpendicular to the first segment and parallel to the piercing post, such that the distal ends of the piercing post and the second portion of the arms are in opposite directions;
capturing optical data from the at least one fractionated blood sample by a photodetector, wherein the photodetector is in communication with a processor;
identifying a location or boundary between individual blood components in the at least one fractionated blood sample by a visual processing algorithm; and,
providing active feedback control based on the location of or boundaries between the individual blood components as determined by the visual processing algorithm, by providing instructions to mechanical means to separate, move and store the components.

2. The method of claim 1 wherein the initial blood component fractionation is achieved by rapid sedimentation or centrifugation.

3. The method of claim 2 , wherein the mechanical separation of the components by the mechanical means occurs either during or after the sedimentation process.

4. The method of claim 3 , wherein the processor determines when the sedimentation process has finished.

5. The method of claim 1 , wherein the processor is additionally in communication with a light source to illuminate the sample to aid in capturing optical data by a photodetector.

6. The method of claim 1 , wherein valves separate the piercing post from each arm.

7. The method of claim 6 , wherein each valve is independently operable.

8. The method of claim 1 , wherein the device can be freely rotated.

9. A device for handling components of a biological sample, wherein the device comprises a sample vessel and a piercing post to receive a sample, such that the sample vessel and the piercing post are in fluid communication, and the piercing post comprises at least a first and second arm extending perpendicular to a length of the sample vessel, wherein,
each of the first and second arms are arranged in opposite direction to each other, wherein each at least first and second arm has an “L” shape, such that each at least first and second arm has a first perpendicular segment, wherein a proximal end of the first perpendicular segment is attached to the piercing post, and a distal end of the first perpendicular segment has a bend between the first perpendicular segment, and a second portion of each arm, wherein the second portion extends from the bend in a direction perpendicular to the first segment and parallel to the piercing post, such that the distal ends of the piercing post and the second portion of the arms are in opposite directions.

10. The device of claim 9 , wherein valves separate the piercing post from each arm.

11. The device of claim 9 , wherein each valve is independently operable.

Assignees

• Canon Virginia Inc

Inventors

• Mikhaiel Nabil
• Sanford Lindsay
• Coughenour Kelly
• Schreiber Jeremy

Classifications

• B01L2300/0672

  Integrated piercing tool · CPC title

• B01L3/523

  with means for closing or opening · CPC title

• B01D17/0214

  with removal of one of the phases · CPC title

• B01D21/286

  Means for gentle agitation for enhancing flocculation · CPC title

• A61M2202/0415

  Plasma · CPC title