Substrate with channels for controlled fluid flow

Patent metadata

Field	Value
Publication number	US-12290806-B2
Application number	US-202217687413-A
Country	US
Kind code	B2
Filing date	Mar 4, 2022
Priority date	Feb 10, 2017
Publication date	May 6, 2025
Grant date	May 6, 2025

Abstract

A substrate structured to define thereon a fluid flow channel and/or a fluid control feature is described. The substrate may additionally comprise a capture zone and/or a test zone, for use as a test strip for determining presence or absence of an analyte of interest, such as an infectious agent or a biomarker. Reagents are deposited in the capture zone and/or test zone as an array of drops.

First claim

It is claimed:

1. A device, comprising:
a laminated structure comprising a nitrocellulose substrate layer provided on a hydrophobic support layer,
a plurality of parallel fluid flow channels, each parallel fluid flow channel separated from an adjacent parallel fluid flow channel by a nitrocellulose substrate-devoid gap exposing the hydrophobic support layer, wherein each substrate-devoid gap forms a side channel that is essentially impermeable to flow of an aqueous fluid;
a single sample receiving zone positioned on the substrate to distribute a portion of a sample deposited thereon into each fluid flow channel in the plurality of parallel fluid flow channels;
a fluid control feature ablated or etched on a surface of the nitrocellulose substrate, the fluid control feature composed of a nitrocellulose substrate-devoid area exposing the hydrophobic support layer, wherein the fluid control feature is configured to guide the portion of sample into one or more of the parallel fluid flow channels; and
a capture zone in each of the plurality of fluid flow channels, wherein the capture zone comprises a reagent comprising a binding member.

2. The device of claim 1 , wherein the plurality of fluid flow channels comprises between 3-50 fluid flow channels.

3. The device of claim 1 , wherein the hydrophobic support layer consists essentially of polyethylene terephthalate, one or more polyesters, and/or silicone.

4. The device of claim 1 , wherein the binding member comprises an immobilized binding member that binds a conjugate comprised of a detectable species and an antibody.

5. The device of claim 4 , wherein the detectable species is an antibody with a label.

6. The device of claim 5 , wherein the label is optically detectable.

7. The device of claim 6 , wherein the optically detectable label is a fluorescent or chemiluminescent marker.

8. The device of claim 6 , wherein the optically detectable label is a non-visually optically detectable label.

9. The device of claim 7 , wherein the detectable species is a europium bead.

10. The device of claim 1 , wherein the fluid control feature is in a flow rate control zone disposed downstream of the sample receiving zone.

11. The device of claim 10 , wherein the flow rate control zone is disposed between the sample receiving zone and the capture zone.

12. The device of claim 1 , wherein the device is an immunoassay device.

Assignees

• Quidel Corp
• Ortho Clinical Diagnostics Inc

Inventors

• Chang Samantha
• Mcclure Jason
• Reed Robert
• Blandy Irene Sinn
• Weiller Robert
• Bobritchi Christian

Classifications

• G01N33/54389 Primary

  with bidirectional or multidirectional lateral flow, e.g. wherein the sample flows from a single, common sample application point into multiple strips, lanes or zones · CPC title

• G01N33/569

  for microorganisms, e.g. protozoa, bacteria, viruses · CPC title

• B01L2400/082

  Active control of flow resistance, e.g. flow controllers · CPC title

• B01L2300/0858

  Side walls · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title