Substrate with channels for controlled fluid flow

Patent metadata

Field	Value
Publication number	US-2018229232-A1
Application number	US-201815893375-A
Country	US
Kind code	A1
Filing date	Feb 9, 2018
Priority date	Feb 10, 2017
Publication date	Aug 16, 2018
Grant date	—

Abstract

A substrate structured to define thereon a fluid flow channel and/or a fluid control feature is described. The substrate may additionally comprise a capture zone and/or a test zone, for use as a test strip for determining presence or absence of an analyte of interest, such as an infectious agent or a biomarker. Reagents are deposited in the capture zone and/or test zone as an array of drops.

First claim

It is claimed:

1 . A device, comprising:
a substrate comprising a sample receiving zone, a destination zone, a fluid pathway extending from the sample receiving zone and the destination zone, and a fluid control feature created in the fluid pathway by exposure of the substrate to a laser, said fluid control feature controlling (i) rate of a fluid flow across the destination zone, and/or (ii) uniformity of rate of flow of a leading edge of a moving fluid across the destination zone.

2 . The device of claim 1 , wherein the fluid control feature is in a flow rate control zone disposed downstream of the sample receiving zone.

3 . The device of claim 2 , wherein the destination zone is a label zone.

4 . The device of claim 3 , wherein the flow rate control zone is disposed between the sample receiving zone and the label zone.

5 . The device claim 1 , wherein the substrate comprises nitrocellulose.

6 . The device of claim 1 , wherein the substrate comprises a laminate of nitrocellulose and a hydrophobic support layer.

7 . The device of claim 1 , wherein the device is an immunoassay device.

8 . A device, comprising:
a substrate; a first fluid flow channel on the substrate, the first fluid flow channel comprising a fluid flow path on the substrate defined and bounded by opposing, substrate-free side channels that are impermeable to fluid flow; and a first fluid flow control feature disposed on the substrate, the first fluid flow control feature controlling (i) rate of a fluid flow in the first fluid flow channel, and/or (ii) uniformity of rate of flow of a leading edge of a moving fluid in the first fluid flow channel.

9 . The device of claim 8 , comprising a second fluid flow channel defined on the substrate by opposing substrate-free side channels, said opposing side walls impermeable to fluid flow.

10 . The device of claim 9 , wherein the second fluid flow channel comprises a second fluid flow control feature, the second fluid control feature configured to control (i) rate of a fluid flow in the second fluid flow channel, and/or (ii) uniformity of rate of flow of a leading edge of a moving fluid in the second fluid flow channel.

11 . The device of claim 9 , wherein said second fluid flow channel has a fluid flow path parallel to the fluid flow path in the first fluid flow channel, wherein fluid in the first fluid flow channel is isolated from fluid in the second fluid flow channel during use of the device by one of the substrate-free side channels.

12 . The device of claim 9 , wherein said second fluid flow channel has a fluid flow path in an opposite direction of the fluid flow path in the first fluid flow channel.

13 . The device of claim 8 , wherein the first fluid flow channel is circular and defines a circular fluid flow path.

14 . An immunoassay device, comprising:
a device according to claim 8 , where the substrate further comprises a sample receiving zone on the substrate, the sample receiving zone positioned to distribute at least a portion of a sample deposited thereon to a fluid flow path comprising a label zone and a capture zone downstream of the label zone, wherein the label zone, the capture zone, or both the label zone and the capture zone are comprised of an n×m array of discrete dots, where n is greater than or equal to one (1) and m is greater than or equal to zero (0), wherein when m is greater than zero each dot in the an n×m array is separated from an adjacent dot by a distance x, and wherein each dot is comprised of a reagent comprising a binding member.

15 . An immunoassay device, comprising:
a substrate comprised of a plurality of fluid flow channels, each fluid flow channel comprising a fluid flow path on the substrate defined and bounded by opposing, substrate-free side channels that are impermeable to fluid flow; a plurality of first fluid flow control features disposed on the substrate, each fluid flow control feature shaped and positioned to control (i) rate of a fluid flow in a fluid flow channel in the plurality of fluid flow channels, and/or (ii) uniformity of rate of flow of a leading edge of a moving fluid in a fluid flow channel in the plurality of fluid flow channels; a single sample receiving zone positioned on the substrate to distribute a portion of a sample deposited thereon to each fluid flow channel in the plurality of fluid flow channels, and a label zone and a capture zone downstream of the label zone in each of the fluid flow paths, wherein the label zone, the capture zone, or both the label zone and the capture zone are comprised of an n×m array of discrete dots, where n is greater than or equal to one (1) and m is greater than or equal to zero (0), wherein when m is greater than zero each dot in the an n×m array is separated from an adjacent dot by a distance x, and wherein each dot is comprised of a reagent comprising a binding member.

16 . The device of claim 15 , wherein the plurality of fluid flow paths comprises between 3-50 fluid flow paths.

17 . The device of claim 15 , wherein the sample receiving zone dispenses sample to each channel in essentially equal amounts and at essentially equal rates.

18 . The device of claim 15 , wherein the substrate comprises a laminate of nitrocellulose and a hydrophobic support layer.

19 . The device of claim 15 , wherein the reagent in the capture zone in each fluid flow path in the plurality of fluid flow paths is an immobilized binding member binds a conjugate comprised of a detectable species and an antibody.

20 . The device claim 19 , wherein the detectable species is an antibody with a label.

21 . The device of claim 20 , wherein the detectable label is a fluorescent or chemiluminescent marker.

22 . The device of claim 19 , wherein the detectable species is a europium bead.

Assignees

• Quidel Corp

Inventors

• Chang Samantha
• Mcclure Jason
• Reed Robert
• Blandy Irene Sinn
• Weiller Robert
• Bobritchi Christian

Classifications

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01L3/5023 Primary

  with a sample being transported to, and subsequently stored in an absorbent for analysis · CPC title

• B01L2300/0858

  Side walls · CPC title

• G01N33/569

  for microorganisms, e.g. protozoa, bacteria, viruses · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title