Fructophilic lactic acid producing bacteria

Patent metadata

Field	Value
Publication number	US-12257274-B2
Application number	US-202117521383-A
Country	US
Kind code	B2
Filing date	Nov 8, 2021
Priority date	May 13, 2019
Publication date	Mar 25, 2025
Grant date	Mar 25, 2025

Abstract

The present invention discloses a novel fructophilic lactic acid producing bacteria Bacillus coagulans strain FF-7 (MTCC 25235) and the process of isolation and characterization of the bacteria.

First claim

We claim:

1. A fructophilic probiotic bacteria Bacillus coagulans MTCC 25235, isolated from honey, wherein the said bacteria is present in the form of an inoculum, freeze-dried powder, fine powder, tablet, capsule, suspension, solution, emulsion, gummy, chewable or edible foods and administered along with accepted excipients, adjuvants, bioavailability enhancers.

2. The fructophilic bacteria as in claim 1 , wherein the said bacteria are isolated from honey using a process comprising steps of:
a) Mixing honey with saline in the ratio of 1:10 w/v to obtain a suspension;
b) Thoroughly mixing the suspension of step a) and providing heat shock at 50-70° C. for 30 minutes for selective isolation of spores;
c) Isolating the bacterial colonies by incubating 1-2 ml of the suspension from step b) in suitable culture media containing fructose for 48 hrs at 35-37° C.;
d) Purifying bacterial isolates by selecting and incubating morphologically distinct colonies in a suitable media containing fructose as carbon source;
e) Identifying the bacterial strain by biochemical analysis and 16S rRNA sequencing as Bacillus coagulans strain FF7 bearing accession no. MTCC 25235.

3. The method fructophilic bacteria as in claim 2 , wherein the honey is selected from the group consisting of, raw honey, filtered honey, acacia honey, alfalfa honey, aster honey, avocado honey, basswood honey, beechwood honey, blueberry honey, bluegum honey, buckwheat honey, clover honey, dandelion honey, eucalyptus honey, fireweed honey, heather honey, ironbark honey, jarrah honey, leatherwood honey, linden honey, macadamia honey, manuka honey, orangeblossom honey, pinetree honey, sourwood honey, sage honey, and tupelo honey.

4. The fructophilic bacteria as in claim 2 , wherein the culture media is selected from the group consisting of MRS (De Man, Rogosa and Sharpe agar), GYA (Glucose Yeast Extract Agar), TSB (Tryptone Soya Broth), Sporulation media and Mueller Hinton Agar.

5. The fructophilic bacteria as in claim 2 , wherein the isolated probiotic strain returns positive for biochemical tests catalase, oxidase, methyl red, voges proskauers, lactose, xylose, maltose, fructose, dextrose, galactose, raffinose, trehalose, melobiose, sucrose, arabinose, mannose, inulin, sodium gluconate, salicin, sorbitol, mannitol, arabitol, methyl glucoside, rhamnose, cellobiose, ONPG, esculin hydrolysis and negative for biochemical tests sorbose malonate utilization, citrate utilization, xylitol, methyl mannoside, melezitose, erythritol, adonitol, inositol, dulcitol, glycerol, blood hemolysis, citrate and indole.

6. The fructophilic bacteria as claim 1 , wherein the optimum pH and temperature for the growth of fructophilic bacteria is 7.5 and 40° C. respectively.

7. The probiotic bacteria as claimed in claim 1 , wherein the said fructophilic probiotic bacteria is bile tolerant, gastric acid resistance and produces lactic acid.

Assignees

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Beede Kirankumar
• Arumugam Sivakumar
• Sami Sabinsa Group Ltd

Inventors

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Beede Kirankumar
• Arumugam Sivakumar

Classifications

• C12Q1/689

  for bacteria · CPC title

• A61K9/2054

  Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose · CPC title

• C12P7/56

  Lactic acid · CPC title

• C12R2001/07

  Bacillus · CPC title

• C12N1/205

  Bacterial isolates · CPC title