Method for killing colorectal cancer cells

Patent metadata

Field	Value
Publication number	US-12220430-B2
Application number	US-202318301505-A
Country	US
Kind code	B2
Filing date	Apr 17, 2023
Priority date	Apr 15, 2021
Publication date	Feb 11, 2025
Grant date	Feb 11, 2025

Abstract

Silver and titanium oxide nanoparticles produced with Fomes fomentarius or Fomitopsis pinicola aqueous extracts and methods for treating bacterial infections or cancer using them.

First claim

The invention claimed is:

1. A method of making TiO 2 nanoparticles, comprising:
drying a Fomitopsis pinicola sample to form a F. pinicola powder,
mixing the F. pinicola powder with water to form a F. pinicola precursor,
sonicating the F. pinicola precursor then centrifuging to obtain a F. pinicola extract;
reacting the F. pinicola extract with titanium isopropoxide to form the TiO 2 nanoparticles; and
isolating and drying the TiO 2 nanoparticles,
wherein upon contacting the TiO 2 nanoparticles with colorectal cancer cells a cell viability of the colorectal cancer cells is reduced to 20% of a cell viability of a control group of colorectal cancer cells not contacted with the TiO 2 nanoparticles,
wherein an amount of the TiO 2 nanoparticles in the contacting with the colorectal cancer cells is 0.5 μg per milliliter of a cell culture of the colorectal cancer cells,
wherein upon contacting the TiO 2 nanoparticles with the colorectal cancer cells a cell nuclei of the colorectal cancer cells undergo nuclear condensation thereby killing a majority of the colorectal cancer cells,
wherein the TiO 2 nanoparticles contain components of the F. pinicola as evident by Fourier transform infrared (FT-IR) spectroscopy absorption peaks at 1414 cm −1 , and 1000 cm −1 corresponding to aliphatic C—N, and aromatic C═N bands, respectively,
wherein the TiO 2 nanoparticles have a further FT-IR spectroscopy absorption peak at 3439 cm −1 corresponding to —OH, and
wherein the TiO 2 nanoparticles have a broadest dimension ranging from 80 to 120 nm.

2. The method of claim 1 , wherein the TiO 2 nanoparticles are agglomerated.

3. The method of claim 1 , wherein the TiO 2 nanoparticles are in a rutile phase and an anatase phase.

4. The method of claim 1 , wherein in the reacting a ratio of the F. pinicola extract to the titanium isopropoxide by volume is 1:10, and
wherein the titanium isopropoxide has a 1 millimolar concentration in water.

5. The method of claim 4 , wherein the colorectal cancer cells are HCT-116 cells.

Assignees

• Univ Imam Abdulrahman Bin Faisal

Inventors

• Rehman Suriya
• Jermy Rabindran

Classifications

• A61K36/07

  Basidiomycota, e.g. Cryptococcus · CPC title

• A61K33/38

  Silver; Compounds thereof · CPC title

• A61K33/08

  Oxides; Hydroxides · CPC title

• A61K9/51

  Nanocapsules; {Nanoparticles; (nanotubes A61K9/0092; polymeric micelles A61K9/1075; polymersomes A61K9/1273; pure drug nanoparticles A61K9/14; drug nanoparticles with adsorbed surface modifiers A61K9/141; conjugates, e.g. between drug and non-active nanoparticles, A61K47/50; preparations for in vivo diagnosis A61K49/00; with radioactive substances A61K51/00)} · CPC title

• A61K9/5192

  Processes · CPC title