Synthesis of silver nanoparticles using fungi

Patent metadata

Field	Value
Publication number	US-9701552-B1
Application number	US-201615338259-A
Country	US
Kind code	B1
Filing date	Oct 28, 2016
Priority date	Oct 28, 2016
Publication date	Jul 11, 2017
Grant date	Jul 11, 2017

Abstract

A method of preparing metal nanoparticles from fungi includes preparing a biomass of fungal cells; providing an aqueous solution including a metal salt; mixing the biomass of fungal cells with the aqueous solution of metal salt; and incubating the resulting mixture at a temperature range of 35° C. to 60° C. to produce the metal nanoparticles.

First claim

We claim:

1. A method of preparing silver nanoparticles from fungi comprising:
preparing a biomass of fungal cells, wherein the fungal cells are obtained from Alternaria pluriseptat;
providing an aqueous solution including a metal salt, wherein the metal salt is silver nitrate (AgNO 3 );
mixing the biomass of fungal cells with the aqueous solution of metal salt to form a mixture; and
incubating the mixture at a temperature ranging from about 35° C. to about 60° C. to produce the silver nanoparticles, wherein the silver nanoparticles have a mean diameter of 200 nm.

2. The method of preparing metal nanoparticles from fungi according to claim 1 , wherein the incubating is performed in the dark for at least 24 hours.

3. The method of preparing metal nanoparticles from fungi according to claim 1 , wherein the incubating occurs at 45° C.

4. The method of preparing metal nanoparticles from fungi according to claim 1 , further comprising isolating the metal nanoparticles from the fungal cells using centrifugation at 10,000 rpm for about 10 minutes.

5. The method of preparing metal nanoparticles from fungi according to claim 1 , wherein the metal nanoparticles are spherical, spheroidal, elongated spherical, rod-shaped, and/or faceted.

6. A method of decontaminating water comprising:
contacting water with an effective amount of the metal nanoparticles produced according to the method of claim 1 for a time sufficient to remove microorganisms or pollutants from the water.

7. The method of decontaminating water according to claim 6 , further comprising adding sodium sulfate to the water.

8. The method of decontaminating water according to claim 6 , wherein the water is sewage water.

Assignees

• Univ King Saud

Inventors

• Ortashi Khalid Mustafa Osman
• Awad Manal Ahmed Gasmelseed
• Hendi Awatif Ahmed
• Abdelaziz Abeer Ramadan Mohamed
• Hendi Ahmed Sameer Ahmed
• Alahmed Abdulhakeem Abdulmuhseen

Classifications

• C12P3/00

  Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)} · CPC title

• C02F1/50 Primary

  by addition or application of a germicide or by oligodynamic treatment {(C02F1/4606, C02F1/467, C02F1/76 take precedence)} · CPC title

• C02F2305/08

  Nanoparticles or nanotubes · CPC title

• A01N63/30

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C02F2303/04

  Disinfection · CPC title