Microfluidic devices and methods for using the devices

Patent metadata

Field	Value
Publication number	US-12208388-B2
Application number	US-201917277608-A
Country	US
Kind code	B2
Filing date	Sep 17, 2019
Priority date	Sep 19, 2018
Publication date	Jan 28, 2025
Grant date	Jan 28, 2025

Abstract

A microfluidic device may include: electrically conductive lower portions; electrically conductive first upper portions that are configured to receive an electrical signal; shielding portions opaque to an incident optical beam, wherein the shielding portions are between the lower portions and first upper portions, and wherein the shielding portions include through openings; and compartment(s) containing filler and markers dispersed in the filler. Each compartment may include lower chamber(s) and upper chamber(s) in fluid communication with each other via the through openings. Each lower chamber may extend between a respective through opening and the lower portions. Each upper chamber may extend between at least one respective through opening and the first upper portions. The markers may be configured to move between the upper chambers and the lower chambers in variable amounts, and may be configured to emit an optical emission beam when illuminated by the incident optical beam.

First claim

The invention claimed is:

1. A microfluidic device, comprising:
lower portions electrically conductive and transparent to an incident optical beam;
first upper portions electrically conductive and configured to receive an electrical signal;
shielding portions opaque to the incident optical beam and arranged between the first upper portions and the lower portions, the shielding portions having one or more through openings; and
one or more compartments containing filler means and markers dispersed in the filler means;
wherein each of the one or more compartments comprises one or more lower chambers and one or more upper chambers in fluid communication with each other via the one or more through openings,
wherein each of the one or more lower chambers extends between a respective through opening and the lower portions,
wherein each of the one or more upper chambers extends between at least one respective through opening and the first upper portions, and
wherein the markers are electrically charged and are configured to move between one of the one or more upper chambers and one of the one or more lower chambers in variable amounts according to an intensity of the electrical signal applied to a respective first upper portion, and are configured to emit an optical emission beam when illuminated in a respective one of the one or more lower chambers by the incident optical beam.

2. The microfluidic device of claim 1 , wherein the markers are configured to emit the optical emission beam through the lower portions.

3. The microfluidic device of claim 1 , further comprising:
second upper portions arranged between contiguous first upper portions;
wherein the second upper portions are transparent to the optical emission beam.

4. The microfluidic device of claim 3 , wherein the markers are configured to emit the optical emission beam through the second upper portions.

5. The microfluidic device of claim 3 , wherein the second upper portions are electrically insulating.

6. The microfluidic device of claim 1 , further comprising:
a plurality of the one or more lower chambers shaped as a plurality of lower cavities extending between a respective through opening of the one or more through openings and the lower portions to form an array of lower cavities.

7. The microfluidic device of claim 6 , further comprising:
a plurality of the one or more upper chambers shaped as a plurality of upper cavities extending between a respective through opening of the one or more through openings and the first upper portions to form an array of upper cavities;
wherein each upper cavity in the array of upper cavities is in fluid communication with a respective lower cavity in the array of lower cavities.

8. The microfluidic device of claim 6 , wherein the one or more upper chambers of a respective compartment of the one or more compartments continuously extends between the shielding portions and the first upper portions so as to be in fluid communication with the plurality of lower cavities via the one or more through openings.

9. The microfluidic device of claim 1 , wherein the filler means is in a form of liquid or gel.

10. The microfluidic device of claim 1 , wherein the markers are selected from among:
fluorophores having predetermined spectral emission, molecular weight, and electrical charge;
quantum dots;
nanorods; and
nanoparticles of predetermined size.

11. The microfluidic device of claim 1 , wherein the first upper portions comprise a plurality of upper electrodes, and
wherein each of the upper electrodes is configured to receive a respective electrical signal.

12. The microfluidic device of claim 1 , wherein the first upper portions are configured to accommodate one or more cells and to receive the electrical signal corresponding to an action potential generated by an excited cell of the one or more cells.

13. An apparatus, comprising:
the microfluidic device of claim 1 ;
an optical source configured to emit the incident optical beam and to direct the incident optical beam toward the lower portions of the microfluidic device; and
an optical detection device configured to receive the optical emission beam emitted by the markers.

14. A method of measuring action potential of a cell using the apparatus of claim 13 , the method comprising:
providing the cell on a respective first upper portion of the first upper portions;
generating the incident optical beam using the optical source; and
receiving the optical emission beam and filtering a predetermined wavelength of the optical emission beam.

15. A method of storing data using the microfluidic device of claim 11 , the method comprising:
receiving a plurality of electrical signals using the plurality of upper electrodes;
generating the incident optical beam using an optical source; and
receiving the optical emission beam and filtering a predetermined wavelength of the optical emission beam.

16. A microfluidic device, comprising:
lower portions that are electrically conductive;
first upper portions that are electrically conductive and configured to receive an electrical signal;
shielding portions opaque to an incident optical beam, wherein the shielding portions are between the lower portions and the first upper portions, and wherein the shielding portions comprise one or more through openings; and
one or more compartments containing filler and markers dispersed in the filler;
wherein each of the one or more compartments comprises one or more lower chambers and one or more upper chambers in fluid communication with each other via the one or more through openings,
wherein each of the one or more lower chambers extends between a respective through opening and the lower portions,
wherein each of the one or more upper chambers extends between at least one respective through opening and the first upper portions, and
wherein the markers are electrically charged and are configured to move between one of the one or more upper chambers and one of the one or more lower chambers in variable amounts, and are configured to emit an optical emission beam when illuminated in a respective one of the one or more lower chambers by the incident optical beam.

17. The microfluidic device of claim 16 , wherein the lower portions are totally transparent to the incident optical beam.

18. The microfluidic device of claim 16 , wherein the lower portions are partly transparent to the incident optical beam.

19. The microfluidic device of claim 16 , wherein the markers are configured to move between the one of the one or more upper chambers and the one of the one or more lower chambers in the variable amounts according to an intensity of the electrical signal applied to the respective first upper portion.

20. The microfluidic device of claim 16 , wherein the markers are configured to move between the one of the one or more upper chambers and the one of the one or more lower chambers in the variable amounts according to a sign of the electrical signal applied to the respective first upper portion.

Assignees

• Fondazione St Italiano Tecnologia
• Univ Degli Studi Genova

Inventors

• De Angelis Francesco
• Barbaglia Andrea
• Dipalo Michele
• Tantussi Francesco

Classifications

• G01N33/5005

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• B01L2300/069

  Absorbents; Gels to retain a fluid · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title