Microfluidic device and methods for using such device

Patent metadata

Field	Value
Publication number	US-2023051647-A1
Application number	US-202117793713-A
Country	US
Kind code	A1
Filing date	Jan 21, 2021
Priority date	Jan 22, 2020
Publication date	Feb 16, 2023
Grant date	—

Abstract

A microfluidic device comprises a lower layer that is electrically conductive and transparent with respect to an incident optical beam, an upper layer, comprising first portions that are electrically conductive and second portions that are electrically insulating, adjacent and alternated to the first ones; a compartment seamlessly extending between the lower layer and the upper layer; the compartment contains a filler medium configured to emit an optical emission beam and markers dispersed in the filler medium, which are electrically charged and are adapted to move inside the compartment in all directions according to the intensity of the electrical signal applied to the first portions, the filler medium is configured to interact with the markers to increase or decrease the intensity of the optical emission beam according to the local concentration of the markers.

First claim

1 . A microfluidic device comprising:
a lower layer that is electrically conductive and transparent with respect to an incident optical beam, an upper layer, comprising
first portions that are electrically conductive and configured to receive an electrical signal, and
second portions that are electrically insulating, the first and second portions being adjacent and alternated between them,
characterised in that it comprises: a compartment interposed between the lower layer and the upper layer throughout the extension of the lower layer and of the upper layer and seamlessly extending between the lower layer and the upper layer, the compartment containing a filler medium configured to emit an optical emission beam when lit by an incident optical beam and markers dispersed in the filler medium, wherein the markers are electrically charged and are adapted to move inside the compartment in all directions in variable amounts according to the intensity of the electrical signal applied to one or more of the first portions, the filler medium being configured to interact with the markers to increase or reduce the intensity of the optical emission beam depending on the local concentration of markers in the compartment.

2 . The microfluidic device according to claim 1 , wherein the first portions are at least partially reflective with respect to the incident optical beam.

3 . The microfluidic device according to claim 1 , wherein the second portions are transparent with respect to the optical emission beam.

4 . The microfluidic device according to claim 1 , wherein the second portions are at least partially reflective with respect to the incident optical beam.

5 . The microfluidic device according to claim 1 , wherein
the first portions comprise a plurality of upper electrodes, each upper electrode being configured to receive a respective electrical signal, the lower layer consists of a single lower electrode.

6 . The microfluidic device according to claim 5 , wherein upper electrodes have a conformation selected from plate, polygonal, spheroidal or a combination thereof.

7 . The microfluidic device according to claim 5 , wherein the upper electrodes and the lower electrode are electrically connected between them in an electric circuit or are floating.

8 . The microfluidic device according to claim 1 , wherein the first portions are spaced between them at a distance between 1 and 100 μm.

9 . The microfluidic device according to claim 1 , wherein the markers are ions of salts of predefined dimensions and/or molecular weight and/or electrical charge and/or hydrodynamic radius.

10 . The microfluidic device according to claim 1 , wherein the filler medium is a substance in solid form.

11 . The microfluidic device according to claim 1 , wherein each first portion is configured to house at least a cell and to receive an electrical signal of the action potential generated by the excited cell.

12 . The microfluidic device according to claim 1 , wherein the first portions have such dimensions as to house one single cell each.

13 . An apparatus comprising:
a microfluidic device according to claim 1 , an optical source configured to emit an incident optical beam towards the lower layer at least at the first portions of the microfluidic device, a detection optical device configured to receive an optical emission beam generated by the filler medium.

14 . A method for measuring the action potential of a cell using an apparatus according to claim 13 , comprising the steps of:
providing a cell on a first portion, generating the incident optical beam by means of an optical source, receiving the optical emission beam and filtering a predefined wavelength of the optical emission beam.

15 . A method for storing data by the apparatus according to claim 13 , wherein the markers move to and from zones defined inside the compartment and underlying the respective first portions, said method comprising the steps of:
generating a plurality of electrical signals on the first portions, generating the incident optical beam by means of an optical source, receiving the optical emission beam and filtering a predefined wavelength of the optical emission beam, associating to each zone a logical number according to the intensity of the optical emission beam coming from the zones.

Assignees

• Fondazione St Italiano Tecnologia

Inventors

• De Angelis Francesco
• Dipalo Michele
• Tantussi Francesco
• Barbaglia Andrea
• Toma Andrea

Classifications

• G01N33/48728

  Investigating individual cells, e.g. by patch clamp, voltage clamp (investigating individual particles in general G01N15/10) · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2400/0415

  electrical forces, e.g. electrokinetic · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title