Method of producing sulfur-containing amino acid or derivative thereof

Patent metadata

Field	Value
Publication number	US-12203117-B2
Application number	US-202017597019-A
Country	US
Kind code	B2
Filing date	Jun 26, 2020
Priority date	Jun 28, 2019
Publication date	Jan 21, 2025
Grant date	Jan 21, 2025

Abstract

A method of producing sulfur-containing amino acids or derivatives of the sulfur-containing amino acids.

First claim

What is claimed is:

1. A method of producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid, the method comprising culturing a genetically modified microorganism in a culture medium including thiosulfate,
wherein the microorganism includes genetic modification to increase an expression level of a protein encoded by ssuABC gene compared to a non-modified microorganism,
wherein the protein encoded by ssuABC gene is a complex of SsuA, SsuB, and SsuC proteins, and wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid is selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine, and
wherein the microorganism has an increase in an expression level of at least one protein selected from the group consisting of SsuA, SsuB, and SsuC proteins compared to a non-modified microorganism.

2. The method of claim 1 , wherein the protein encoded by ssuABC gene has thiosulfate transporter activity.

3. The method of claim 1 , wherein the microorganism has an increase in an expression level of SsuA, SsuB, and SsuC proteins compared to a non-modified microorganism.

4. The method of claim 1 , wherein the SsuA protein includes an amino acid sequence having at least 80% of homology with an amino acid sequence of SEQ ID NO: 43.

5. The method of claim 1 , wherein the SsuB protein includes an amino acid sequence having at least 80% of homology with an amino acid sequence of SEQ ID NO: 44.

6. The method of claim 1 , wherein the SsuC protein includes an amino acid sequence having at least 80% of homology with an amino acid sequence of SEQ ID NO: 45.

7. The method of claim 1 , wherein the microorganism is a microorganism belonging to the genus Corynebacterium sp. or the genus Escherichia sp.

8. The method of claim 1 , further comprising recovering the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid from the microorganism or the culture medium.

9. The method of claim 1 , wherein genetic modification to increase the expression level of the protein is achieved by i) increasing a copy number of a polynucleotide encoding the protein in a cell, ii) replacing an expression regulatory region of a polynucleotide encoding the protein with a sequence with stronger activity, iii) modifying an initiation codon or 5′-UTR of a polynucleotide encoding the protein, iv) modifying a nucleotide sequence on a chromosome to enhance the activity of the protein, v) introducing a foreign polynucleotide expressing the activity of the protein or a codon optimized variant polynucleotide of the polynucleotide encoding the protein, or vi) a combination thereof.

10. A microorganism producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid and including genetic modification to increase an expression level of a protein encoded by ssuABC gene compared to a non-modified microorganism, wherein the protein encoded by ssuABC gene is a complex of SsuA, SsuB, and SsuC proteins, and wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid is selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine, and wherein the microorganism produces the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid using thiosulfate as a sulfur source.

11. The microorganism of claim 10 , wherein genetic modification to increase the expression level of the protein is achieved by i) increasing a copy number of a polynucleotide encoding the protein in a cell, ii) replacing an expression regulatory region of a polynucleotide encoding the protein with a sequence with stronger activity, iii) modifying an initiation codon or 5′-UTR of a polynucleotide encoding the protein, iv) modifying a nucleotide sequence on a chromosome to enhance the activity of the protein, v) introducing a foreign polynucleotide expressing the activity of the protein or a codon optimized variant polynucleotide of the polynucleotide encoding the protein, or vi) a combination thereof.

12. A composition for producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid,
wherein the composition comprises: a microorganism including genetic modification to increase an expression level of a protein encoded by ssuABC gene compared to a non-modified microorganism, or a culture thereof; and thiosulfate;
wherein the protein encoded by ssuABC gene is a complex of SsuA, SsuB, and SsuC proteins, and wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid is selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine.

Assignees

• Cj Cheiljedang Corp

Inventors

• Choi Sol
• Kim Hee Ju
• Rho Jin Ah
• Lee Jin Nam
• Lee Han Hyoung
• Lee Sun Young
• Kim Sang Jun
• Shim Jihyun

Classifications

• C12N15/77 Primary

  for Corynebacterium; for Brevibacterium · CPC title

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C07K14/34

  from Corynebacterium (G) · CPC title

• C07K14/245

  Escherichia (G) · CPC title

• C12R2001/28

  Micrococcus glutamicus {; Corynebacterium glutamicum} · CPC title