Promoter and uses thereof

Patent metadata

Field	Value
Publication number	US-10273491-B2
Application number	US-201615547398-A
Country	US
Kind code	B2
Filing date	Jan 15, 2016
Priority date	Jan 29, 2015
Publication date	Apr 30, 2019
Grant date	Apr 30, 2019

Abstract

Provided are a novel promoter and a method of producing a target product using the same.

First claim

The invention claimed is:

1. A synthetic promoter comprising the nucleotide sequence of SEQ ID NO: 1.

2. An expression regulatory sequence, comprising the promoter of claim 1 .

3. A vector comprising the expression regulatory sequence of claim 2 and a target gene that is operatively linked to the expression regulatory sequence.

4. A host cell comprising the vector of claim 3 .

5. The host cell according to claim 4 , wherein the host cell is a bacterial cell belonging to the genus Corynebacterium or the genus Escherichia.

6. A method of producing a target product, the method comprising:
culturing the host cell of claim 4 ; and
recovering the target product from the host cell or a culture medium including the cultured host cell.

7. The method of claim 6 , wherein the target product is an amino acid.

8. A method of producing a target product, the method comprising:
culturing the host cell of claim 5 ; and
recovering the target product from the host cell or a culture medium including the cultured host cell.

Assignees

• Cj Cheiljedang Corp

Inventors

• Lee Seung Bin
• Bae Hyun Ae
• Lee Ji Hye
• Yang Young Lyeol

Classifications

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12N15/77 Primary

  for Corynebacterium; for Brevibacterium · CPC title

• C07K14/34

  from Corynebacterium (G) · CPC title