Ribonucleic acid purification
US-2016024140-A1 · Jan 28, 2016 · US
RNA polymerase variants
US12195778B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12195778-B2 |
| Application number | US-202318448856-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 11, 2023 |
| Priority date | Aug 18, 2017 |
| Publication date | Jan 14, 2025 |
| Grant date | Jan 14, 2025 |
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- Title
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Abstract
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The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of performing an in vitro transcription (IVT) reaction, comprising combining a deoxyribonucleic acid (DNA) template with a T7 ribonucleic acid (RNA) polymerase variant, nucleoside triphosphates, and buffer under conditions that result in the production of to produce an RNA transcript, wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 1 modified to comprise an amino acid substitution at position. 2. The method of claim 1 , wherein the amino acid substitution is selected from the group consisting of alanine, isoleucine, leucine, methionine, lysine, glutamine, and glutamate. 3. The method of claim 2 , wherein the amino acid substitution is alanine. 4. The method of claim 1 , wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 95% identity to SEQ ID NO:1. 5. The method of claim 1 , wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 98% identity to SEQ ID NO:1. 6. The method of claim 1 , wherein the T7 RNA polymerase comprises an additional C-terminal amino acid. 7. The method of claim 6 , wherein the additional C-terminal amino acid comprises glycine (G). 8. The method of claim 1 , wherein the T7 RNA polymerase comprises two additional C-terminal amino acids. 9. The method of claim 8 , wherein the two additional C-terminal amino acids comprise the same type of amino acid. 10. The method of claim 8 , wherein the two additional C-terminal amino acids comprise two different types of amino acids. 11. The method of claim 1 , wherein the amino acid substitution at position S43 is alanine (S43A). 12. The method of claim 11 , wherein the RNA polymerase variant comprises the amino acid sequence of SEQ ID NO: 2.
Assignees
Inventors
Classifications
- C12N9/1247Primary
DNA-directed RNA polymerase (2.7.7.6) · CPC title
[RNA-polymerase-subunit] kinase (2.7.11.23) · CPC title
Methyl · CPC title
with an inverted bond, e.g. a cap structure · CPC title
reducing unwanted side-effects · CPC title
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- What does patent US12195778B2 cover?
- The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
- Who is the assignee on this patent?
- Modernatx Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1247. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 14 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).