Compositions for facilitating biological stain removal

Patent metadata

Field	Value
Publication number	US-12195704-B2
Application number	US-202318114051-A
Country	US
Kind code	B2
Filing date	Feb 24, 2023
Priority date	Jun 21, 2010
Publication date	Jan 14, 2025
Grant date	Jan 14, 2025

Abstract

A liquid bioactive composition is provided that includes an enzyme with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein the enzyme in the liquid bioactive coating material is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from a substrate.

First claim

The invention claimed is:

1. A composition for facilitating biological stain removal comprising a liquid bioactive coating material comprising:
a surfactant;
a compound selected from the group consisting of ammonia, a water miscible alcohol having 1 to 6 carbon atoms, and combinations thereof, and
an enzyme, said enzyme capable of degrading a biological stain component, wherein said enzyme is a thermolysin-like protease, an amylase, or a lipase.

2. The composition of claim 1 , wherein said thermolysin-like protease is an active extracellular portion of bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus.

3. The composition of claim 1 , wherein said liquid bioactive coating material includes a protease at a specific activity in excess of 20,000 U/g.

4. The composition of claim 1 , wherein the pH of said liquid bioactive coating material is greater than 5.0.

5. The composition of claim 1 , wherein said enzyme is in solution in said liquid bioactive coating material.

6. The composition of claim 1 , wherein said liquid bioactive coating material comprises said alcohol, said alcohol present at less than 0.8% by weight.

7. The composition of claim 1 , wherein said liquid bioactive coating material comprises said ammonia.

8. The composition of claim 1 , wherein said composition comprises said alcohol, and said alcohol is methanol.

9. A method of facilitating the removal of a biological stain on a substrate or a coating comprising:
providing the liquid coating material of claim 1 ;
contacting said liquid coating material to a biological stain to thereby facilitate removal of the biological stain.

10. The method of claim 9 , wherein said liquid bioactive coating material includes a protease at a specific activity in excess of 20,000 U/g.

11. The method of claim 9 , wherein the pH of the liquid bioactive coating material is greater than 5.0.

12. The method of claim 9 , wherein said enzyme is in solution in said liquid bioactive coating material.

13. The method of claim 9 , wherein said liquid bioactive coating material comprises said water miscible alcohol, said alcohol present at less than 0.8% by weight.

14. The method of claim 9 , wherein said bioactive liquid coating material comprises said ammonia.

15. The method of claim 9 , wherein said biological stain is an insect stain.

16. The method of claim 9 , wherein said biological stain is present on a substrate.

17. The method of claim 9 , wherein said substrate is glass.

Assignees

• Toyota Motor Corp
• Toyota Eng & Mfg North America

Inventors

• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan

Classifications

• C11D2111/18

  Glass; Plastics · CPC title

• D06M16/003

  with enzymes or microorganisms · CPC title

• C12Y304/24027

  Thermolysin (3.4.24.27) · CPC title

• C11D3/43

  Solvents · CPC title

• C11D3/201

  linear · CPC title