Nanobarcode for controlling cell adhesion and differentiation of stem cells

Patent metadata

Field	Value
Publication number	US-12180505-B2
Application number	US-202117246376-A
Country	US
Kind code	B2
Filing date	Apr 30, 2021
Priority date	May 22, 2020
Publication date	Dec 31, 2024
Grant date	Dec 31, 2024

Abstract

Disclosed are a nanobarcode for controlling adhesion and differentiation of stem cells and a method of controlling adhesion and differentiation of stem cells by using nanobarcodes. The method of controlling adhesion and differentiation of stem cells of the present invention may efficiently control adhesion and differentiation of stem cells in vivo or in vitro by tuning periodicity and sequences of a ligand peptide (RGD) of a nanobarcode.

First claim

What is claimed is:

1. A nanobarcode for controlling adhesion and differentiation of stem cells, the nanobarcode comprising:
a nanobarcode in which a first segment including iron (Fe) and a second segment including gold (Au) are repeatedly formed; and
an integrin ligand peptide bound to the second segment of the nanobarcode, wherein the integrin ligand peptide comprises a uniform distribution in a monolayer, and the nanobarcode comprises a density of 0.0235 to 0.0277 per square micrometer (μm 2 ), and the stem cells adhere to the second segment.

2. The nanobarcode of claim 1 , wherein the nanobarcode is provided in a rod shape satisfying Equation 1 or Equation 2 below,
[L(M 1 M 2 )q]  [Equation 1]

[L(M 1 M 2 M 2 M 1 )q]  [Equation 2]

herein, M 1 is the first segment, M 2 is the second segment, q is the number of times of the repetition of the first and second segments, and L is lengths of the first and second segments.

3. The nanobarcode of claim 2 , wherein Equations 1 and 2 are any one of [30(M 1 M 2 ) 10 ], [75(M 1 M 2 ) 4 ], [75(M 1 M 2 M 2 M 1 ) 2 ], [150(M 1 M 2 ) 2 ], [150(M 1 M 2 M 2 M 1 ) 1 ], and [300(M 1 M 2 ) 1 ].

4. The nanobarcode of claim 1 , wherein each of the first segment and the second segment is provided in a rod shape, and a length of the first segment is the same as a length of the second segment.

5. The nanobarcode of claim 1 , wherein the first segment has a structure in which a carboxylate is substituted.

6. The nanobarcode of claim 1 , wherein the nanobarcode has a rod shape having a circular cross section with a diameter of 50 nm to 100 nm and a length of 200 to 1,000 nm.

7. The nanobarcode of claim 1 , wherein the integrin ligand peptide includes a thiolated integrin ligand peptide, and has a structure in which a thiol group of the integrin ligand peptide is chemically bound to the second segment.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Kang Heemin
• Kim Young Keun
• Min Sunhong
• Jeon Yoo Sang
• Choi Hyojun

Classifications

• C07K5/0817

  the first amino acid being Arg · CPC title

• C12N2501/998

  Proteins not provided for elsewhere · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• C12N5/0662 Primary

  Stem cells · CPC title