Bone marrow microfluidic devices and methods for preparing and using the same

Patent metadata

Field	Value
Publication number	US-12180447-B2
Application number	US-202318343514-A
Country	US
Kind code	B2
Filing date	Jun 28, 2023
Priority date	Mar 24, 2017
Publication date	Dec 31, 2024
Grant date	Dec 31, 2024

Abstract

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

First claim

What is claimed is:

1. A method of preparing a microfluidic device, the method comprising:
providing a microfluidic device having a first microchannel, a second microchannel, and a porous membrane separating the first microchannel from the second microchannel;
providing a matrix within the first microchannel, the matrix comprising CD34+ bone marrow progenitor cells;
perfusing media through the second microchannel;
producing cells differentiated from said CD34+ bone marrow progenitor cells, said differentiated cells comprising erythroid lineage cells.

2. The method of claim 1 , wherein the matrix is a fibrin gel.

3. The method of claim 2 , wherein said fibrin gel comprises aprotinin.

4. The method of claim 1 , further comprising seeding the second microchannel with endothelial cells.

5. The method of claim 1 , wherein the first microchannel forms a closed channel.

6. The method of claim 1 , wherein said matrix further comprises stromal cells.

7. The method of claim 1 , wherein there is no flow of media through said first microchannel.

8. The method of claim 7 , wherein media in said second microchannel can migrate through said membrane into said first microchannel.

Assignees

• Harvard College
• Massachusetts Gen Hospital

Inventors

• Chou David Benson
• Leijten Liliana S Teixeira Moreira
• Rech Arianna
• Novak Richard
• Ingber Donald E
• Milton Yuka
• Frismantas Viktoras
• Levy Oren

Classifications

• G01N33/5008

  for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics · CPC title

• C12N2533/90

  Substrates of biological origin, e.g. extracellular matrix, decellularised tissue · CPC title

• C12N2533/56

  Fibrin; Thrombin · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12N2502/28

  Vascular endothelial cells · CPC title