Organ mimic device with microchannels and methods of use and manufacturing thereof

Patent metadata

Field	Value
Publication number	US-2016046897-A1
Application number	US-201514922840-A
Country	US
Kind code	A1
Filing date	Oct 26, 2015
Priority date	Jul 16, 2008
Publication date	Feb 18, 2016
Grant date	—

Abstract

System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

First claim

What is claimed is:

1 . A method, comprising: a) providing a microfluidic device having a body, the body including a fluid channel and a membrane, the fluid channel containing stem cells; and b) applying fluid to said stem cells through said fluid channel.

2 . The method of claim 1 , wherein said membrane is positioned along a plane within said fluid channel to partition the channel into a first microchannel and a second microchannel.

3 . The method of claim 1 , further comprising c) allowing differentiation cues to reach the stem cells thereby differentiating the stem cells to different cell types.

4 . The method of claim 1 , wherein said stem cells are human embryonic stem cells.

5 . The method of claim 1 , wherein said stem cells are induced pluripotent stem cells.

6 . The method of claim 1 , wherein said stem cells are stem cells isolated from adult tissues.

7 . The method of claim 1 , wherein said stem cells are mesenchymal stem cells.

8 . The method of claim 1 , wherein said stem cells are hematopoietic stem cells.

9 . The method of claim 1 , wherein said stem cells are stem cell of osteoblasts.

10 . The method of claim 1 , wherein said stem cells are stem cell for neurons.

11 . The method of claim 1 , wherein said stem cells are stem cell for olfactory neurons.

12 . The method of claim 1 , wherein said stem cells are stem cell for spermatocytes.

13 . The method of claim 1 , wherein said culture fluid is serum free.

14 . The method of claim 1 , wherein said method further comprises c) contacting said cells with an agent and d) measuring the response of said cells to said agent.

15 . The method of claim 14 , wherein said agent is a chemical agent.

16 . The method of claim 14 , wherein said agent is a biological agent.

17 . The method of claim 14 , wherein said agent is a pharmacological agent.

18 . A microfluidic device comprising a body, the body including a fluid channel and a membrane, the fluid channel containing stem cells in fluid.

19 . The microfluidic device of claim 18 , wherein said membrane is positioned along a plane within said fluid channel to partition the channel into a first microchannel and a second microchannel.

20 . The microfluidic device of claim 18 , wherein said stem cells are human embryonic stem cells.

21 . The microfluidic device of claim 18 , wherein said stem cells are induced pluripotent stem cells.

22 . The microfluidic device of claim 18 , wherein said stem cells are stem cells isolated from adult tissues.

23 . The microfluidic device of claim 18 , wherein said stem cells are mesenchymal stem cells.

24 . The microfluidic device of claim 18 , wherein said stem cells are hematopoietic stem cells.

25 . The microfluidic device of claim 18 , wherein said stem cells are stem cell of osteoblasts.

26 . The microfluidic device of claim 18 , wherein said stem cells are stem cell for neurons.

27 . The microfluidic device of claim 18 , wherein said stem cells are stem cell for olfactory neurons.

28 . The microfluidic device of claim 18 , wherein said stem cells are stem cell for spermatocytes.

Assignees

• Childrens Medical Center

Inventors

• Ingber Donald E
• Huh Dongeun

Classifications

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• C12N5/0654

  Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title