Methods and compositions for extending shelf life of plant products

Patent metadata

Field	Value
Publication number	US-12152282-B2
Application number	US-202318308862-A
Country	US
Kind code	B2
Filing date	Apr 28, 2023
Priority date	Sep 24, 2012
Publication date	Nov 26, 2024
Grant date	Nov 26, 2024

Abstract

The invention provides compositions and methods related to selective inhibition of PPO11 and use for improving shelf life of a plant or parts thereof. In accordance with the invention, for example, compositions for topical application to a plant or part thereof, are provided that can reduce browning of the plant or part thereof to extend shelf life.

First claim

What is claimed is:

1. A plant having reduced expression or lacking expression of a PPO11 gene or ortholog thereof, produced by a method comprising the steps of:
a) obtaining a plurality of plants at least one of which comprises a mutation that results in reduced expression or no detectable expression of the PPO11 gene relative to other members of the plurality of plants;
b) screening the plurality of plants to identify the mutation that results in said reduced expression or no detectable expression of the PPO11 gene;
c) selecting said plant that comprises said mutation,
wherein the PPO11 gene has at least 95% sequence identity to SEQ ID NO:9.

2. The plant of claim 1 , wherein the plurality of plants are obtained by random mutagenesis.

3. The plant of claim 1 , wherein the plurality of plants are transgenic plants.

4. The plant of claim 1 , wherein the plurality of plants comprise 10, 100, or 1000 or more plants.

5. The plant of claim 1 , wherein the plurality of plants are varieties of the same species of plants.

6. The plant of claim 1 , wherein screening for decreased expression or lacking expression of the PPO11 gene comprises determining an abundance of PPO11 RNA, determining PPO11 activity, or determining abundance of a protein or RNA encoded by SEQ ID NO:9.

7. The plant of claim 1 , wherein the method further comprises crossing said plant with reduced expression or lacking expression of the PPO11 gene to a different plant.

8. The plant of claim 1 , wherein said plurality of plants are lettuce plants.

Assignees

• Seminis Vegetable Seeds Inc

Inventors

• Feikert Michele
• Froman Byron
• Garvey Graeme S
• Kelly Leo
• Waycott William

Classifications

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• A01N65/12

  Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• A23B7/155

  Microorganisms; Enzymes {; Antibiotics} · CPC title

• C12N15/8266

  Abscission; Dehiscence; Senescence · CPC title