Methods for identifying plants with extended shelf life

Patent metadata

Field	Value
Publication number	US-10106859-B2
Application number	US-201615343061-A
Country	US
Kind code	B2
Filing date	Nov 3, 2016
Priority date	Sep 24, 2012
Publication date	Oct 23, 2018
Grant date	Oct 23, 2018

Abstract

The invention provides compositions and methods related to selective inhibition of PPO11 and use for improving shelf life of a plant or parts thereof. In accordance with the invention, for example, compositions for topical application to a plant or part thereof, are provided that can reduce browning of the plant or part thereof to extend shelf life.

First claim

What is claimed is:

1. A method for identifying plants having reduced expression or lacking expression of a PPO11 gene, comprising:
a) obtaining a plurality of plants;
b) screening the plurality of plants for expression of a PPO11 gene having at least 95% sequence identity to SEQ ID NO:9 in which expression is positively correlated with browning or reduced shelf life of a processed product of the plant;
c) selecting one or more screened plants comprising decreased expression of the PPO11 gene relative to expression of the PPO11 gene in a reference plant of the same crop species, a parent plant, or an otherwise isogenic plant, and which displays browning or reduced shelf life.

2. The method of claim 1 , wherein the plurality of plants are obtained by random mutagenesis to obtain plants having reduced expression or lacking expression of the PPO11 gene.

3. The method of claim 1 , wherein the plurality of plants are transgenic plants.

4. The method of claim 1 , wherein the plurality of plants comprise 10, 100, or 1000 or more plants.

5. The method of claim 1 , wherein the plurality of plants are varieties of the same species of plants.

6. The method of claim 1 , wherein screening for expression of the PPO11 gene comprises determining an abundance of PPO11 RNA, determining PPO11 activity, or determining abundance of a protein or RNA encoded by SEQ ID NO:9.

7. The method of claim 1 , further comprising crossing the one or more plants with reduced expression of PPO11 to a different plant.

8. A method of lettuce breeding comprising:
a) identifying a first lettuce plant according to the method of claim 1 ; and
b) crossing the first lettuce plant with itself or a second lettuce plant to produce progeny lettuce plants having reduced expression or lacking expression of the PPO11 gene.

9. The method of claim 1 , wherein said plants are lettuce plants.

Assignees

• Seminis Vegetable Seeds Inc

Inventors

• Feikert Michele
• Froman Byron
• Garvey Graeme S
• Kelly Leo
• Waycott William

Classifications

• A23B7/155

  Microorganisms; Enzymes {; Antibiotics} · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12N9/0059

  Catechol oxidase (1.10.3.1), i.e. tyrosinase · CPC title