Microfluidic valve and method of making same

Patent metadata

Field	Value
Publication number	US-12128405-B2
Application number	US-202016925762-A
Country	US
Kind code	B2
Filing date	Jul 10, 2020
Priority date	Nov 14, 2006
Publication date	Oct 29, 2024
Grant date	Oct 29, 2024

Abstract

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

First claim

What is claimed:

1. A method of making a microfluidic valve, the method comprising:
propelling via air a quantity of thermally responsive substance into an inlet hole of a microfluidic cartridge, wherein the microfluidic cartridge comprises a single substrate comprising a microfluidic channel on a first side of the substrate and the inlet hole on the second side of the substrate, wherein the microfluidic cartridge comprises a laminate layer attached to the first side of the substrate;
maintaining a temperature of the microfluidic cartridge with a heater so that the thermally responsive substance flows by capillary action into a microfluidic channel in communication with the inlet hole, wherein the thermally responsive substance stops within the microfluidic channel to form the microfluidic valve; and
covering the inlet hole with a label on the second side of the substrate after the thermally responsive substance stops.

2. The method of claim 1 , wherein covering the inlet hole comprises covering two or more inlet holes.

3. The method of claim 1 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in a sample lane of the microfluidic cartridge.

4. The method of claim 1 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in different sample lanes of the microfluidic cartridge.

5. The method of claim 1 , wherein covering the inlet hole comprises covering the microfluidic cartridge with the label comprising a layer of plastic.

6. The method of claim 1 , wherein covering the inlet hole comprises covering the microfluidic cartridge with the label comprising a layer comprising a pressure sensitive adhesive.

7. The method of claim 1 , wherein propelling a quantity of thermally responsive substance comprises propelling a quantity of thermally responsive substance to the bottom of the inlet hole.

8. The method of claim 1 , wherein propelling a quantity of thermally responsive substance comprises propelling a molten thermally responsive substance.

9. A method of making a microfluidic valve, the method comprising:
positioning a microfluidic cartridge relative to a heater, wherein the microfluidic cartridge comprises an inlet hole, a loading channel in communication with the inlet hole, a flow channel along which a fluid passes, and a junction between the loading channel and the flow channel, wherein the loading channel comprises a first section between the inlet hole and a shoulder, and wherein the loading channel comprises a second section between the shoulder and the junction;
propelling via air a quantity of thermally responsive substance to the bottom of the inlet hole;
applying heat from the heater to the thermally responsive substance when the thermally responsive substance is in the microfluidic cartridge so that the thermally responsive substance stays in a molten state immediately after the thermally responsive substance is dispensed, wherein the thermally responsive substance flows entirely into the second section, and wherein the quantity of the thermally responsive substance is equal to or is less than the volume of the second section; and
covering the inlet hole after the thermally responsive substance is dispensed.

10. The method of claim 9 , wherein the thermally responsive substance flows by capillary action into the loading channel in communication with the inlet hole.

11. The method of claim 10 , wherein the loading channel in communication with the inlet hole facilitates motion of the thermally responsive substance.

12. The method of claim 10 , wherein the first section has a larger cross-section than the second section.

13. The method of claim 9 , further comprising heating and maintaining a temperature of the thermally responsive substance prior to propelling the quantity of thermally responsive substance.

14. The method of claim 9 , further comprising covering the inlet hole with a label after the thermally responsive substance is dispensed.

15. The method of claim 9 , further comprising inspecting the valve.

16. The method of claim 9 , further comprising applying a label.

17. A method of making a microfluidic valve, the method comprising:
propelling via air a quantity of thermally responsive substance into the inlet hole of a microfluidic cartridge, wherein the microfluidic cartridge comprises a microfluidic channel in communication with the inlet hole, a flow channel along which a fluid passes, and a junction between the microfluidic channel and the flow channel;
heating the microfluidic cartridge with one or more heating elements;
maintaining a temperature of the microfluidic cartridge so that the thermally responsive substance flows by capillary action into the microfluidic channel in communication with the inlet hole, wherein the microfluidic channel comprises one or more bends along the length of the microfluidic channel from the inlet hole to the junction; and
covering the inlet hole after the thermally responsive substance is propelled.

18. The method of claim 17 , wherein the microfluidic channel comprises a first section and a second section, and wherein the first section has a larger cross-section than the second section.

19. The method of claim 18 , wherein the first section is closer to the inlet hole.

20. The method of claim 9 , wherein the quantity is between 60 nl and 90 nl.

Assignees

• Handylab Inc

Inventors

• Handique Kalyan
• Parunak Gene
• Haley Cecelia

Classifications

• B01L2300/087

  Multiple sequential chambers · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B81C1/00087

  Holes · CPC title

• F16K2099/0084

  Chemistry or biology, e.g. "lab-on-a-chip" technology · CPC title