Microfluidic chip features for optical and thermal isolation

Patent metadata

Field	Value
Publication number	US-9234236-B2
Application number	US-201414508173-A
Country	US
Kind code	B2
Filing date	Oct 7, 2014
Priority date	May 14, 2009
Publication date	Jan 12, 2016
Grant date	Jan 12, 2016

Abstract

A microfluidic chip includes microfluidic channels, elements for thermally and optically isolating the microfluidic channels, and elements for enhancing the detection of optical signal emitted from the microfluidic channels. The thermal and optical isolation elements may comprise barrier channels interposed between adjacently-arranged pairs of microfluidic channels for preventing thermal and optical cross-talk between the adjacent microfluidic channels. The isolation element may alternatively comprise reflective film embedded in the microfluidic chip between the adjacent microfluidic channels. The signal enhancement elements comprise structures disposed adjacent to the microfluidic channels that reflect light passing through or emitted from the microfluidic channel in a direction toward a detector. The structures may comprise channels or a faceted surface that redirects the light by total internal reflection or reflective film material embedded in the microfluidic chip.

First claim

The invention claimed is:

1. A microfluidic device comprising:
at least one microfluidic channel having fluorescent indicators;
wherein the micro fluidic device comprises a reflecting surface, separate from the at least one microfluidic channel, configured to cause light passing through or emanating from the at least one microfluidic channel having fluorescent indicators toward said reflecting surface to be redirected by total internal reflection.

2. The microfluidic device of claim 1 , wherein said reflecting surface comprises surface facets oriented at angles with respect to each other.

3. The microfluidic device of claim 2 , wherein the facets are oriented at an angle of approximately 45 degrees with respect to each other.

4. The microfluidic device of claim 1 , wherein at least a portion of the micro fluidic device comprising a reflecting surface is located only beneath the at least one microfluidic channel.

5. The microfluidic device of claim 1 , wherein the material forming said microfluidic device comprises glass.

6. The microfluidic device of claim 2 , wherein the surface facets function as signal enhancement structures.

7. The microfluidic device of claim 1 , wherein the reflecting surface is opposite a flat surface of the microfluidic chip.

Assignees

• Canon Us Life Sciences Inc

Inventors

• Thomas Bradley S
• Coursey Johnathan S
• Hasson Kenton C
• Liang Hongye

Classifications

• B01L2300/18

  Means for temperature control · CPC title

• B01L7/52

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• Y10T436/11

  Automated chemical analysis · CPC title

• B01L2300/0858

  Side walls · CPC title