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US-2024172743-A1 · May 30, 2024 · US
Cell cryopreservation composition and cryopreservation method
US12127553B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12127553-B2 |
| Application number | US-202217664194-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 19, 2022 |
| Priority date | Jan 31, 2017 |
| Publication date | Oct 29, 2024 |
| Grant date | Oct 29, 2024 |
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- Title
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Abstract
Official abstract text for this publication.
A cell cryopreservation method including adding a 1 volume % of a composition comprising 0.01 wt % to 20 wt % of a sophorose lipid to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells; and cryopreserving the cell culture medium, wherein the composition improves cell viability after cryopreservation compared to cells that are cryopreserved with a similar composition that does not contain the sophorose lipid.
First claim
Opening claim text (preview).
What is claimed is: 1. A cell cryopreservation method comprising: adding a 1 volume % of a composition comprising 0.01 wt % to 20 wt % of a sophorose lipid to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells; and cryopreserving the cell culture medium, wherein the composition improves cell viability after cryopreservation compared to cells that are cryopreserved with a similar composition that does not contain the sophorose lipid. 2. The method according to claim 1 , wherein the composition further comprises 5 wt % to 10 wt % of dimethyl sulfoxide (DMSO). 3. The method according to claim 2 , wherein a 10 volume % to 99 volume % of the composition is added to the cells. 4. The method according to claim 1 , wherein the composition further comprises 1 wt % to 50 wt % of a polyhydric alcohol. 5. The method of claim 4 , wherein the polyhydric alcohol comprises at least one of glycerin, ethylene glycol, and propylene glycol. 6. The method of claim 4 , wherein the composition does not contain dimethyl sulfoxide (DMSO). 7. The method of claim 4 , wherein the polyhydric alcohol is glycerin, and the cells are stem cells. 8. The method of claim 4 , wherein the stem cells are mesenchymal stem cells.
Assignees
Inventors
Classifications
- A01N1/125Primary
Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators · CPC title
Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood · CPC title
Bone marrow mesenchymal stem cells (BM-MSC) · CPC title
Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title
DMSO · CPC title
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- What does patent US12127553B2 cover?
- A cell cryopreservation method including adding a 1 volume % of a composition comprising 0.01 wt % to 20 wt % of a sophorose lipid to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells; and cryopreserving the cell culture medium, wherein the composition improves cell viability after cryopreservation compared to cells that are cryopreserved with a similar…
- Who is the assignee on this patent?
- Saraya Co Ltd, Univ Osaka
- What technology area does this patent fall under?
- Primary CPC classification A01N1/125. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Oct 29 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).