Method for constructing halophilic nitrogen assimilation microbiome

Patent metadata

Field	Value
Publication number	US-12122699-B2
Application number	US-202217590475-A
Country	US
Kind code	B2
Filing date	Feb 1, 2022
Priority date	Feb 1, 2021
Publication date	Oct 22, 2024
Grant date	Oct 22, 2024

Abstract

The present invention provides a method for constructing a halophilic nitrogen assimilation microbiome, and belongs to the technical fields of microorganisms as well as environmental governance and restoration. According to the present invention, a strain of Psychrobacter aquimaris A4N01 is screened from marine sediments; the strain can assimilate ammonia nitrogen to synthesize organic nitrogen, and simultaneously convert organic matters and phosphorus. A halophilic nitrogen assimilation microbiome developed by the strain has good nutrient conversion removal capacity and settling performance, has wide tolerance to salinity, and can be used in high-salinity wastewater treatment, saline-alkali soil fertilization, etc., so as to realize recycling of nutrient elements such as nitrogen and phosphorus in high-salinity environments. The method is simple in operation and low in costs and is environmentally friendly, therefore having good practical application value.

First claim

What is claimed is:

1. A method for constructing a halophilic nitrogen assimilation microbiome, the method comprising:
inoculating Psychrobacter aquimaris A4NO1 into a bioreactor containing high-salinity wastewater having a salinity of not less than 2% (w/w), and operating the bioreactor with operation cycles, each operation cycle comprising four steps of generating influent, aeration, settling and generating effluent to obtain a halophilic nitrogen assimilation microbiome, wherein
the Psychrobacter aquimaris A4N01 has been deposited in China Center for Type Culture Collection on Jan. 20, 2021 with a biological deposit number of CCTCC NO: M 2021120.

2. The method according to claim 1 , wherein the
inoculated strain an inoculation amount of the Psychrobacter aquimaris A4N01 is controlled to be not less than 5 g/L; and
the high-salinity wastewater is simulated high-salinity wastewater, the simulated high-salinity wastewater has a salinity of—not less than 2% (w/w) 3%-7% (w/w), and the simulated high-salinity wastewater is prepared with aged seawater.

3. The method according to claim 1 , wherein the simulated high-salinity wastewater comprises the following ingredients: 0.8 g/L glucose, 0.5 g/L sodium acetate, 0.55 g/L ammonium chloride, 0.14 g/L dipotassium phosphate and 0.25 mg/L peptone, and the simulated high-salinity wastewater is prepared with aged seawater with a salinity of 3.3%.

4. The method according to claim 1 , wherein:
the bioreactor is a sequencing batch bioreactor; and
the sequencing batch bioreactor is operated in a continuous mode, high-salinity wastewater with a salinity of 3-7% is used as influent, DO is controlled to be maintained at 2-3 mg/L through aeration, and a carbon-nitrogen ratio of the influent is not less than 10.

5. The method according to claim 4 , wherein an operation cycle of the sequencing batch bioreactor is 8 h, comprising 5 min of generating influent, 450 min of aeration, 15 min of settling and 10 min of generating effluent without mud discharging.

Assignees

• Univ Shandong

Inventors

• Zhou Weizhi
• Zhang Mengru

Classifications

• C02F3/1263

  Sequencing batch reactors [SBR] · CPC title

• C12N1/205

  Bacterial isolates · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C02F2103/08

  Seawater, e.g. for desalination · CPC title

• C02F2103/005

  Black water originating from toilets · CPC title