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US-11913006-B2 · Feb 27, 2024 · US
Bioconversion of levulinic acid in genetically engineered hosts
US12116609B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12116609-B2 |
| Application number | US-202117159850-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 27, 2021 |
| Priority date | Sep 19, 2017 |
| Publication date | Oct 15, 2024 |
| Grant date | Oct 15, 2024 |
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- Title
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Abstract
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Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of making 2-butanone, the method comprising culturing a genetically modified host cell transformed to contain and express a heterologous recombinant expression vector comprising at least one promoter operably linked to at least three nucleotide sequences selected from the group consisting of lvaA, lvaB, lvaC, lvaD, and lvaE, wherein lvaA has a nucleotide sequence having at least 90% sequence identity to SEQ. ID. NO: 1, lvaB has a nucleotide sequence having at least 90% sequence identity to SEQ. ID. NO: 3, lvaC has a nucleotide sequence having at least 90% sequence identity to SEQ. ID. NO: 5, lvaD has a nucleotide sequence having at least 90% sequence identity to SEQ. ID. NO: 7, and lvaE has a nucleotide sequence having at least 90% sequence identity to SEQ. ID. NO: 9, in a medium containing levulinic acid, under conditions and for a time wherein at least a portion of the levulinic acid is catabolized by action of the host cell into 2-butanone. 2. The method of claim 1 , wherein the heterologous recombinant expression vector comprises the at least one promoter operably linked to at least four nucleotide sequences selected from the group consisting of lvaA, lvaB, lvaC, lvaD, and lvaE. 3. The method of claim 1 , wherein the heterologous recombinant expression vector comprises the at least one promoter operably linked to lvaA, lvaB, lvaC, lvaD, and lvaE.
Assignees
Inventors
Classifications
Ketones · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
Increasing the copy number of the vector · CPC title
Carboxylic ester hydrolases (3.1.1) · CPC title
Acetyl-CoA C-acetyltransferase (2.3.1.9) · CPC title
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- What does patent US12116609B2 cover?
- Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.
- Who is the assignee on this patent?
- Wisconsin Alumni Res Found
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1029. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 15 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).