Data processing module of a biological test system

Patent metadata

Field	Value
Publication number	US-12100291-B2
Application number	US-202117303496-A
Country	US
Kind code	B2
Filing date	May 31, 2021
Priority date	Dec 30, 2019
Publication date	Sep 24, 2024
Grant date	Sep 24, 2024

Abstract

A test system includes a test container array including a plurality of test containers, and a plurality of electrodes integrated into the test container array. The test system further includes a plurality of drive-sense circuits coupled to the plurality of electrodes. When enabled, and when the test container contains a content, the set of drive-sense circuits transmit a set of electrode signals on the set of electrodes and generate a set of sensed signals. The test system further includes a processing module that includes a bandpass filter circuit operable to convert a sensed signal of the set of sensed signals to a filtered signal of a set of filtered signals and a frequency interpreter operable to convert the set of filtered signals into a set of impedance values, where the set of impedance values are representative of electrical characteristics of the content.

First claim

What is claimed is:

1. A test system comprises:
a test container array including a plurality of test containers;
a plurality of electrodes integrated into the test container array, wherein a set of electrodes of the plurality of electrodes is integrated into a test container of the test container array;
a plurality of drive-sense circuits coupled to the plurality of electrodes, wherein a set of drive-sense circuits of the plurality of drive-sense circuits is coupled to the set of electrodes, and wherein, when enabled, and when the test container contains a content, the set of drive-sense circuits are operable to:
transmit a set of electrode signals on the set of electrodes, wherein an electrode signal of the set of electrode signals includes a transmit signal component having a frequency; and
generate a set of sensed signals, wherein a sensed signal of the set of sensed signals includes a frequency component corresponding to the transmit signal component having the frequency; and

a processing module coupled to the plurality of drive-sense circuits, wherein the processing module includes:
a bandpass filter circuit having a set of bandpass filters coupled to the set of drive-sense circuits, wherein the bandpass filter circuit is operable to convert the set of sensed signals into a set of filtered signals, wherein a bandpass filter of the set of bandpass filters passes signals in a bandpass region corresponding to the frequency component to convert a sensed signal of the set of sensed signals to a filtered signal of the set of filtered signals; and
a frequency interpreter coupled to the bandpass filter circuit, wherein the frequency interpreter is operable to:
convert the set of filtered signals into a set of impedance values, wherein the set of impedance values are representative of electrical characteristics of the content.

2. The test system of claim 1 further comprises:
a multiplexor coupled to the set of drive-sense circuits, wherein the multiplexor is operable to:
receive one or more control signals from the processing module; and
select, in accordance with a timing scheme, a transmitting drive-sense circuit of the set of drive-sense circuits based on the one or more control signals, wherein the transmitting drive-sense circuit transmits the electrode signal including the transmit signal component having the frequency, wherein the electrode signal is generated based on a reference signal including the frequency; and

wherein non-transmitting drive-sense circuits of the set of drive-sense circuits are operable to generate the set of sensed signals, wherein each sensed signal of the set of sensed signals includes the frequency component corresponding to the transmit signal component having the frequency.

3. The test system of claim 1 , wherein the content includes:
a solution;
one or more biological cells;
a portion of one or more biological cells; and
a testing substance.

4. The test system of claim 1 , wherein the electrical characteristics include one or more of:
position;
impedance;
size;
shape;
movement;
density;
excitability; and
potential.

5. The test system of claim 1 , wherein an impedance value of the set of impedance values includes one or more of:
an actual impedance value;
a relative impedance value; and
a difference impedance value.

6. The test system of claim 1 , wherein the processing module is further operable to:
generate an impedance map based on the set of impedance values and with respect to positioning of the set of electrodes, wherein the impedance map is representative of electrical characteristics of the content.

7. The test system of claim 6 further comprises:
when enabled, and when a second content is added to the test container, the set of drive-sense circuits are operable to:
transmit a second set of electrode signals on the set of electrodes;
generate a second set of sensed signals; and

the processing module is further operable to:
interpret the second set of sensed signals as a second set of impedance values;
generate a second impedance map based on the second set of impedance values and with respect to the positioning of the set of electrodes, wherein the second impedance map is representative of electrical characteristics of the content and second content; and
compare the impedance map and second impedance map to determine electrical characteristics of the second content.

8. The test system of claim 7 , wherein the processing module is further operable to:
determine the content is a biological material, and the second content is a solution.

9. The test system of claim 8 , wherein the solution includes one or more of:
a saline solution;
a preservative; and
a cell culture solution.

10. A test system comprises:
a test container array including a plurality of test containers;
a plurality of electrodes integrated into the test container array, wherein a set of electrodes of the plurality of electrodes is integrated into a test container of the test container array;
a plurality of drive-sense circuits coupled to the plurality of electrodes, wherein a set of drive-sense circuits of the plurality of drive-sense circuits is coupled to the set of electrodes, and wherein, when enabled, and when the test container contains a content, the set of drive-sense circuits is operable to:
transmit a set of electrode signals on the set of electrodes, wherein an electrode signal of the set of electrode signals includes a transmit signal component having a frequency; and
generate a set of sensed signals, wherein a sensed signal of the set of sensed signals includes at least one frequency component corresponding to at least one transmit signal transmitted by at least one other drive-sense circuit of the set of drive-sense circuits; and

a processing module coupled to the plurality of drive-sense circuits, wherein the processing module includes:
a set of bandpass filter circuits coupled to the set of drive-sense circuits, wherein a bandpass filter circuit of the set of bandpass filter circuits includes a set of bandpass filters, wherein a bandpass filter circuit of the set of bandpass filter circuits is operable to convert the set of sensed signals to a set of filtered signals, wherein a bandpass filter of the set of bandpass filters passes signals in a bandpass region corresponding to a frequency component of the at least one frequency component to produce a filtered signal of the set of filtered signals; and
a set of frequency interpreters coupled to the set of bandpass filter circuits, wherein the set of frequency interpreters is operable to:
convert sets of filtered signals into sets of impedance values, wherein the sets of impedance values are representative of electrical characteristics of the content.

11. The test system of claim 10 , wherein the content includes:
a solution;
one or more biological cells;
a portion of one or more biological cells; and
a testing substance.

12. The test system of claim 10 , wherein the electrical characteristics include one or more of:
position;
impedance;
size;
shape;
movement;
density;
excitability; and
potential.

13. The test system of claim 10 , wherein an impedance value of the sets of impedance values includes one or more of:
an actual impedance value;
a relative impedance value; and
a difference impedance value.

14. The test system of claim 10 , wherein the processing module is further operable to:
generate an impedance map based on the sets of impedance values and with respect to positioning of the set of electrodes, wherein the impedance map is representative of e

Assignees

• Sigmasense Llc

Inventors

• Van Ostrand Daniel Keith
• Seger Jr Richard Stuart
• Morrison Gerald Dale
• Gray Patrick Troy
• Huynh Phuong
• Markison Timothy W
• Healy Patricia M

Classifications

• G01N33/4836 Primary

  using multielectrode arrays · CPC title

• G01N27/028 Primary

  Circuits therefor (measuring impedance per se G01R27/02) · CPC title

• G01D21/02

  Measuring two or more variables by means not covered by a single other subclass · CPC title

• B01L2300/0663

  Whole sensors · CPC title

• B01L2300/0645

  Electrodes · CPC title