Electrophoresis method, electrophoresis system, and container vessel for electrophoresis

Patent metadata

Field	Value
Publication number	US-12099031-B2
Application number	US-201917259016-A
Country	US
Kind code	B2
Filing date	Jun 19, 2019
Priority date	Jul 13, 2018
Publication date	Sep 24, 2024
Grant date	Sep 24, 2024

Abstract

A support body includes a concentrating portion, which concentrates an object, between a first end portion and a second end portion. When an area of a cut section of a flow path when cut at a predetermined position in a first direction along a plane orthogonal to the first direction is a flow path cross-sectional area, a flow path cross-sectional area of the concentrating portion is smaller than a flow path cross-sectional area in a first region closer to a first end portion side than the concentrating portion, and a flow path cross-sectional area of a second region closer to the second end portion side than the concentrating portion is larger than the flow path cross-sectional area of the concentrating portion.

First claim

The invention claimed is:

1. An electrophoresis method for causing a sample containing a biological substance to move inside a support body to separate the sample and the biological substance from each other, the method comprising:
a support body preparation step of providing the support body that includes a first end portion on a first end portion side and a second end portion on a second end portion side that is on the other side of the support body, and forms a flow path of the sample in a first direction between the first end portion and the second end portion;
a sample loading step of loading the sample with respect to the support body; and
a voltage application step of applying voltage to perform electrophoresis by forming a potential difference in the flow path and thus to cause the biological substance to move in the flow path in the first direction towards the second end portion side,
wherein the support body includes a concentrating portion, which concentrates the biological substance, between the first end portion and the second end portion, and
when an area of a cut section of the flow path when cut at a predetermined position in the first direction along a plane orthogonal to the first direction is a flow path cross-sectional area, a flow path cross-sectional area of the concentrating portion is smaller than a flow path cross-sectional area in a first region closer to the first end portion side than the concentrating portion, and a flow path cross-sectional area of a second region closer to the second end portion side than the concentrating portion is larger than the flow path cross-sectional area of the concentrating portion,
wherein a defining surface that defines the flow path cross-sectional area has:
a first portion,
a concentrating portion forming portion which forms the concentrating portion that concentrates the biological substance between the first end portion and the second end portion, and
a second portion closer to the second end portion side than the concentrating portion forming portion, wherein:
(a) the second portion of the defining surface has a second surface that, starting from the concentrating portion forming portion, extends in a second direction orthogonal to the first direction in a manner that widens the flow path cross-sectional area through which the biological substance passes during the electrophoresis, and when viewed from a third direction orthogonal to the first and second directions, the first portion of the defining surface has a first surface that, starting from the concentrating portion forming portion, extends in a straight line towards the first end portion side in or parallel to the first direction, or
(b) the second portion of the defining surface has the second surface that, starting from the concentrating portion forming portion, extends in a fourth direction that forms an angle of larger than 90° with respect to the first direction and extends towards the first end portion side that widens the flow path cross-sectional area through which the biological substance passes during the electrophoresis;

wherein the concentrating portion forming portion is spaced apart from the first end portion and the second end portion in the first direction, and
wherein the concentrating portion forming portion defines a top, and the first portion of the defining surface and the second portion of the defining surface are connected at the top in a bent state.

2. The electrophoresis method according to claim 1 ,
wherein an insulator defines the flow path cross-sectional area to form the concentrating portion.

3. The electrophoresis method according to claim 1 ,
wherein the flow path is widened in a boundary portion between the concentrating portion and the second region in a direction intersecting the first direction.

4. The electrophoresis method according to claim 1 ,
wherein the flow path cross-sectional area of the first region is decreased as the first region approaches the concentrating portion from the first end portion side.

5. The electrophoresis method according to claim 1 ,
wherein in the sample loading step, the sample is loaded in a sample loading portion that extends to the second end portion side as the sample loading portion extends away from a center position in the second direction orthogonal to the first direction.

6. An electrophoresis system that causes a sample containing a biological substance to move inside a support body to separate the sample and the biological substance from each other, the system comprising:
the support body that includes a first end portion on a first end portion side and a second end portion on a second end portion side that is on the other side of the support body, and forms a flow path of the sample in a first direction between the first end portion and the second end portion; and
a voltage application unit configured to apply a voltage to perform electrophoresis by forming a potential difference in the flow path and thus to cause the biological substance of the sample, which is loaded on the first end portion side, to move in the flow path in the first direction towards the second end portion side,
wherein the support body includes a concentrating portion, which concentrates the biological substance, between the first end portion and the second end portion, and
when an area of a cut section of the flow path when cut at a predetermined position in the first direction along a plane orthogonal to the first direction is a flow path cross-sectional area, a flow path cross-sectional area of the concentrating portion is smaller than a flow path cross-sectional area in a first region closer to the first end portion side than the concentrating portion, and a flow path cross-sectional area of a second region closer to the second end portion side than the concentrating portion is larger than the flow path cross-sectional area of the concentrating portion,
a defining surface that defines the flow path cross-sectional area has:
a first portion,
a concentrating portion forming portion which forms the concentrating portion that concentrates the biological substance between the first end portion and the second end portion, and
a second portion closer to the second end portion side than the concentrating portion forming portion, wherein:
(a) the second portion of the defining surface has a second surface that, starting from the concentrating portion forming portion, extends in a second direction orthogonal to the first direction in a manner that widens the flow path cross-sectional area through which the biological substance passes during the electrophoresis, and when viewed from a third direction orthogonal to the first and second directions, the first portion of the defining surface has a first surface that, starting from the concentrating portion forming portion, extends in a straight line towards the first end portion side in or parallel to the first direction, or
(b) the second portion of the defining surface has the second surface that, starting from the concentrating portion forming portion, extends in a fourth direction that forms an angle of larger than 90° with respect to the first direction and extends towards the first end portion side that widens the flow path cross-sectional area through which the biological substance passes during the electrophoresis;

wherein the concentrating portion forming portion is spaced apart from the first end portion and the second end portion in the first direction, and
wherein the concentrating portion forming portion defines a top, and the first portion of the defining surface and the second portion of the defining surface are connected at the top in a bent state.

7. The electrophoresis system according to claim 6 ,
wherein an insulato

Assignees

• Hamamatsu Photonics Kk

Inventors

• Hashimoto Yu
• Kazami Sayaka

Classifications

• G01N27/453

  Cells therefor · CPC title

• G01N27/44704 Primary

  Details; Accessories · CPC title