Hyper efficient separations device

Patent metadata

Field	Value
Publication number	US-11090660-B2
Application number	US-201716324324-A
Country	US
Kind code	B2
Filing date	Aug 10, 2017
Priority date	Aug 10, 2016
Publication date	Aug 17, 2021
Grant date	Aug 17, 2021

Abstract

The present technology relates to improved device and methods of use of insulator-based dielectrophoresis. This device provides a multi-length scale element that provides enhanced resolution and separation. The device provides improved particle streamlines, trapping efficiency, and induces laterally similar environments. Also provided are methods of using the device.

First claim

What is claimed is:

1. A hyper-resolution insulator-based dielectrophoresis device for separating an analyte from a fluid, the device comprising:
a fluid flow channel disposed on a substrate, the fluid flow channel being defined by a first wall, a second wall spaced from the first wall, at least one fluid inlet, and at least one fluid outlet, the first wall including an insulating flow structure extending toward the second wall thereby defining a constriction in the fluid flow channel between the first wall and the second wall, the insulating flow structure comprising a base section and a plurality of projections extending from the base section toward the second wall;
electrodes in electrical communication with the at least one fluid inlet and the at least one fluid outlet of the fluid flow channel, wherein the electrodes are positioned to generate a spatially non-uniform electric field across the insulating flow structure of the fluid flow channel to exert a dielectrophoretic force on particles suspended in the fluid within the fluid flow channel, and
a power supply connected to each of the electrodes to generate an electric field within the fluid flow channel,
wherein the base section is elliptically shaped and the plurality of projections are elliptically shaped.

2. The device of claim 1 , wherein each projection has a first end at a first junction with the base section and a second end at a second junction with the base section, a length from the first end to the second end being in the range of 50 nm-100 μm.

3. The device of claim 1 , wherein the base section extends from an upstream junction with the first wall to a downstream junction with the first wall, and the plurality of projections only extend from the base section over a portion of a distance from the upstream junction to the downstream junction.

4. The device of claim 3 , wherein the plurality of projections only extend from the base section over one half or less of the distance from the upstream junction to the downstream junction.

5. The device of claim 1 , wherein:
the second wall includes a second insulating flow structure extending toward the first wall thereby further defining the constriction in the fluid flow channel between the first wall and the second wall, the second insulating flow structure comprising a second base section and a second plurality of projections extending from the second base section toward the first wall.

6. The device of claim 1 , wherein the fluid flow channel is a microchannel.

7. The device of claim 1 , wherein the analyte is a cell.

8. The device of claim 7 , wherein the cell is a human cell.

9. The device of claim 7 , wherein the cell is a bacterial cell.

10. The device of claim 1 , wherein the analyte is a crystal.

11. The device of claim 1 , wherein the analyte is a protein.

12. The device of claim 1 , wherein the analyte is a microorganism.

13. The device of claim 1 , wherein the analyte is a virus.

14. The device of claim 1 , wherein the device provides improved particle streamlines.

15. The device of claim 1 , wherein the device provides enhanced analyte separation and improved resolution.

16. The device of claim 1 , wherein the device reduces or eliminates extraneous trapping zones.

17. The device of claim 1 , wherein the device provides a high ∇| | 2 as to provide enhanced resolution of analytes.

18. A method of separating a first analyte from at least one second analyte, the method comprising:
(a) providing the device of claim 1 ; and
(b) transporting a fluid including the first analyte and the second analyte from the fluid inlet through the constriction in the fluid flow channel toward the fluid outlet.

Assignees

• Univ Arizona State
• Nat Institutes Of Health Nih U S Dept Of Health And Human Services Dhhs U S Government

Inventors

• Hayes Mark
• Crowther Claire
• Jones Paul

Classifications

• B01L2400/0424

  Dielectrophoretic forces · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• G01N15/1484

  microstructural devices · CPC title

• G01N27/447

  using electrophoresis · CPC title

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title