EWOD device with sensing apparatus

Patent metadata

Field	Value
Publication number	US-12066467-B2
Application number	US-202017767640-A
Country	US
Kind code	B2
Filing date	Oct 22, 2020
Priority date	Oct 25, 2019
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

An electro-wetting on dielectric (EWOD) device, comprises first and second substrates defining a fluid chamber therebetween, a plurality of electro-wetting electrodes on the first substrate, and at least one first electrode and at least two second electrodes on the second substrate. The device further includes a current sensor for sensing a difference between (1) a first current flowing between the first electrode and one of the second electrodes via a first fluid package in the fluid chamber of the EWOD device and (2) a second current flowing between the first electrode and another of the second electrodes via a second fluid package in the fluid chamber of the EWOD device.

First claim

The invention claimed is:

1. An electro-wetting on dielectric (EWOD) device, comprising:
first and second substrates defining a fluid chamber therebetween; a plurality of electro-wetting electrodes on the first substrate;
at least one first electrode and at least two second electrodes on the second substrate;

and
a current sensor for sensing a difference between (1) a first current flowing between the first electrode and one of the second electrodes via a first fluid package in the fluid chamber of the EWOD device and (2) a second current flowing between the first electrode and another of the second electrodes via a second fluid package in the fluid chamber of the EWOD device.

2. A device as claimed in claim 1 , wherein the current sensor is a differential current sensor receiving as inputs the first current and the second current.

3. A device as claimed in claim 1 and further comprising at least one third electrode on the second substrate.

4. A device as claimed in claim 1 and comprising a fluid droplet in electrical contact with the first electrode and the one of the second electrodes, the fluid droplet constituting the first fluid package.

5. A device as claimed in claim 3 and comprising a fluid droplet in electrical contact with the first electrode and the one of the second electrodes, the fluid droplet constituting the first fluid package, and wherein the droplet is in electrical contact with the third electrode.

6. A device as claimed in claim 1 and comprising first and second fluid droplets with a droplet interface bilayer therebetween, the first droplet being in electrical contact with the first electrode and the second droplet being in electrical contact with one second electrode, the first and second fluid droplets constituting the first fluid package.

7. A device as claimed in claim 1 , the first electrode extends in a first direction and the one of the second electrodes and the another of the second electrodes extend substantially parallel to one another, and extend in a second direction that is crossed with the first direction.

8. A device as claimed in claim 7 wherein the first electrode and the second electrodes are defined in a common conductive layer.

9. A device as claimed in claim 7 wherein a guard electrode is provided between the first electrode and the second electrodes.

10. A device as claimed in claim 9 wherein the first electrode and the second electrodes are defined in a common conductive layer, and the guard electrode is defined in the common conductive layer.

11. A device as claimed in claim 1 , wherein a plurality of first electrodes are provided on the second substrate, the first electrodes extending substantially parallel to one another in a first direction;
and wherein the second electrodes extend substantially parallel to one another, and extend in a second direction crossed with the first direction.

12. A device as claimed in claim 11 wherein the first electrodes are defined in a first conductive layer and the second electrodes are defined in a second conductive layer.

13. A device as claimed in claim 1 wherein a hydrophobic coating is provided over the electrodes on the second substrate, and wherein apertures are provided in the hydrophobic coating to define regions of electrical contact between the fluid packages and the electrodes.

14. A device as claimed in claim 13 , and comprising a fluid droplet, wherein the diameter of an aperture in the hydrophobic coating is selected to be less than 50% of the diameter of the fluid droplet so as not to disrupt the macroscale hydrophobic surface property of the hydrophobic coating.

15. A method of sensing electrical properties of a fluid package in an electro-wetting on dielectric (EWOD) device, the EWOD device having first and second substrates defining a fluid chamber therebetween, a plurality of electro-wetting electrodes on the first substrate, and at least one bias electrode and at least two sensing electrodes on the second substrate, the method comprising:
applying a first bias voltage to a first bias electrode and applying a second bias voltage to a second bias electrode;
sensing a first current flowing between the first bias electrode and one of the sensing electrodes via a first fluid package electrically coupled to the first bias electrode and the one of the sensing electrodes; and
sensing a second current flowing between the second bias electrode and another of the sensing electrodes via a second fluid package electrically coupled to the second bias electrode and the another of the sensing electrodes.

16. A method as claimed in claim 15 wherein the first fluid package comprises at least one reaction constituent;
wherein the second fluid package is a reference fluid package;
and wherein the method further comprises monitoring the progress of a reaction in the first fluid package by comparing variation with time of electrical properties of the first fluid package with variation with time of electrical properties of the second fluid package.

17. A method as claimed in claim 15 wherein the first fluid package is a fluid droplet and the second fluid package is a second fluid droplet.

18. A method as claimed in claim 15 wherein the first fluid package comprises first and second fluid droplets having a first droplet interface bilayer therebetween, and the second fluid package comprises third and fourth fluid droplets having a second droplet interface bilayer therebetween, the first droplet electrically coupled to the first bias electrode, the second droplet electrically coupled to the one of the sensing electrodes, the third droplet electrically coupled to the second bias electrode, and the fourth droplet electrically coupled to the another of the sensing electrodes.

19. A method as claimed in claim 15 wherein the first bias electrode is electrically continuous with the second bias electrode to form a common bias electrode, and wherein applying the first bias voltage to the first bias electrode and applying the second bias voltage to the second bias electrode comprises applying a common bias voltage to the common bias electrode.

20. A method as claimed in claim 15 and further comprising, before applying the first bias voltage to the first bias electrode and applying the second bias voltage to the second bias electrode:
selectively actuating the electro-wetting electrodes to move a first fluid package in the fluid chamber of the EWOD device to be electrically coupled to a first bias electrode and one of the sensing electrodes; and
selectively actuating the electro-wetting electrodes to move a second fluid package in the fluid chamber of the EWOD device to be adjacent a second bias electrode and another of the sensing electrodes.

Assignees

• Sharp Life Science Eu Ltd

Inventors

• Brown Christopher James
• Hadwen Benjamin James

Classifications

• B01L2400/0427

  Electrowetting · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L2300/0663

  Whole sensors · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L3/502792 Primary

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title