Optical flow cell

Patent metadata

Field	Value
Publication number	US-12066375-B2
Application number	US-201716464759-A
Country	US
Kind code	B2
Filing date	Nov 30, 2017
Priority date	Nov 30, 2016
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.

First claim

The invention claimed is:

1. An optical flow cell comprising:
a housing forming a circumferentially enclosed and elongated fluid channel arranged along a first axis;
an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel; and
a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel,
wherein the housing comprises at least a first part, a second part, and a seal located between the first and the second part, wherein an interface between the first part and the second part is contoured,
wherein the first part and the second part together form the circumferentially enclosed and elongated fluid channel, the first part comprising a portion of the fluid channel with an open side positioned along the elongated fluid channel in a lengthwise direction of the first part, the open side of the first part oriented so that the portion of the fluid channel is not circumferentially enclosed and the second part configured to close the open side and thereby circumferentially enclose the fluid channel,
wherein the seal comprises a seal body extending along an interface between the first part and the second part, and
wherein the seal body has a continuous contoured surface conformed to the interface between the first part and the second part.

2. The optical flow cell according to claim 1 , wherein the first light guide comprises an exit surface, where light is emitted, arranged adjacent to the first side wall of the fluid channel, and wherein the second light guide comprises an entrance surface, where the emitted light is received, arranged adjacent to a second opposing side wall of the fluid channel, wherein the exit surface and the entrance surface is separated by a distance.

3. The optical flow cell according to claim 1 , wherein the seal encloses edges of opposing surfaces of the first and second parts and/or the seal comprises a first and second bore arranged on the second axis, wherein the first bore is arranged to allow the first light guide to protrude through the first bore and the second light guide is arranged to protrude through the second bore.

4. The optical flow cell according to claim 1 , wherein the first light guide is enclosed in a first connector part and the second light guide is enclosed in a second connector part.

5. The optical flow cell according to claim 4 , further comprising:
a first fastener arranged to fasten or secure the first connector part to the housing; and
a second fastener arranged to fasten or secure the second connector part to the housing.

6. The optical flow cell according to claim 1 , wherein the first light guide and second light guide are formed by cutting a continuous light guide along the first axis and/or cutting the continuous light guide in a motion perpendicular to the first axis, such that the first and second light guides are separated by a desired path length and/or distance.

7. The optical flow cell according to claim 1 , wherein the first axis extends along a first inner surface of the first part and a first inner surface of the second part.

8. The optical flow cell according to claim 1 , wherein the continuous contoured surface retains its conformation (i) when free of the optical flow cell and (ii) when positioned along the interface between the first part and the second part.

9. A method of producing the optical flow cell according to claim 1 , the method comprising:
inserting a continuous light guide into an optical flow cell housing along a second axis;
removing a portion of the continuous light guide to form a first light guide having an exit surface, where light is emitted, and a second light guide having an entrance surface, where the emitted light is received;
wherein the portion have a length equal to a distance such that the exit surface and the entrance surface after removing the portion is separated by the distance.

10. The method according to claim 9 , wherein removing a portion of the continuous light guide is performed by cutting the continuous light guide adjacent to a first side wall of the fluid channel and cutting the continuous light guide adjacent to a second opposing side wall of the fluid channel.

11. The method according to claim 9 , wherein removing a portion of the continuous light guide is performed in a motion along the first axis and perpendicular to the second axis.

12. The method according to claim 9 , wherein removing a portion of the continuous light guide is performed in a motion perpendicular to the first axis and perpendicular to the second axis.

13. The method according to claim 9 , wherein removing a portion of the continuous light guide is performed using a diamond saw or laser ablation.

14. A method performed by a measuring device comprising the optical flow cell, according to claim 1 , the method comprising:
obtaining environment data indicative an environment the optical flow cell has been subjected to;
measuring a light absorption value of the optical flow cell indicative of absorption of light emitted from an exit surface of a first light guide to an exit surface of a second light guide, optionally wherein the exit surface and the entrance surface is separated by a nominal distance when the optical flow cell is subjected to a nominal environment; and
generating a compensated light absorption value based on the light absorption value and a compensation function dependent on the environment data.

15. The method according to claim 14 , wherein the compensation function compensates for variations of the distance.

16. The method of claim 14 , wherein the environment data is indicative of one or more of: temperature of the optical flow cell; ambient temperature;
the optical flow cell being subjected to gamma irradiation; and
the optical flow cell being subjected to autoclaving.

17. A measuring device configured for compensating a light absorption value measured in an optical flow cell, the device comprising:
an optical flow cell according to claim 1 ;
a light generator configured to emit light within a bandwidth to the first light guide;
an absorption value generator configured to receive reference light from the light generator and received light from the optical flow cell;
a flow cell control unit, the unit comprising a processor, and a memory, said memory containing instructions executable by said processor, whereby said flow cell control unit is operative and/or configured to perform a method comprising:
inserting a continuous light guide into an optical flow cell housing along a second axis;
removing a portion of the continuous light guide to form a first light guide having an exit surface, where light is emitted, and a second light guide having an entrance surface, where the emitted light is received;
wherein the portion have a length equal to a distance such that the exit surface and the entrance surface after removing the portion is separated by the distance; and
wherein removing a portion of the continuous light guide is performed in a motion perpendicular to the first axis and perpendicular to the second axis.

Assignees

• Cytiva Sweden Ab

Inventors

• Ehring Hanno
• Lundkvist Mats
• Bergman David Carl Martin
• Marcstrom Andreas

Classifications

• G01N21/03

  Cuvette constructions · CPC title

• G01N2201/08

  Optical fibres; light guides · CPC title

• G01N2201/0668

  Multiple paths; optimisable path length · CPC title

• G01N21/85

  Investigating moving fluids or granular solids · CPC title

• G01N21/0303

  Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence) · CPC title