Lov-D acyltransferase mediated acylation
US-10428360-B2 · Oct 1, 2019 · US
Lov-D acyltransferase mediated acylation
US12060592B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12060592-B2 |
| Application number | US-202017089498-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 4, 2020 |
| Priority date | Sep 30, 2009 |
| Publication date | Aug 13, 2024 |
| Grant date | Aug 13, 2024 |
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- Title
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Abstract
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Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
First claim
Opening claim text (preview).
What is claimed is: 1. A non-naturally occurring variant LovD polypeptide having at least two-fold greater acyltransferase activity than the wild-type Aspergillus terreus acyltransferase of SEQ ID NO: 2, which comprises a variant amino acid sequence of SEQ ID NO: 2 that includes a mutation at position 157 and from 1 to 30 additional mutations, as compared to SEQ ID NO: 2. 2. The non-naturally occurring variant LovD polypeptide of claim 1 , which has at least 10-fold greater acyltransferase activity than the wild-type A. terreus acyltransferase of SEQ ID NO: 2. 3. The non-naturally occurring variant LovD polypeptide of claim 1 , that includes the following additional mutations: A123P, N191S/G, A247S and L361M. 4. The non-naturally occurring variant LovD polypeptide of claim 3 , further comprising one or more additional mutations selected from: A9V, K26E, L192I, R250K, G275S, Q297E/G, and A383V. 5. The non-naturally occurring variant LovD polypeptide of claim 4 , wherein the one or more additional mutations are selected from: A9V, K26E, and G275S.
Assignees
Inventors
Classifications
Genes encoding for enzymes or proenzymes · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
using catalysts, e.g. selective catalysts · CPC title
Acyltransferases (2.3) · CPC title
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title
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- What does patent US12060592B2 cover?
- Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P17/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 13 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).