Lov-D acyltransferase mediated acylation
US-9399785-B2 · Jul 26, 2016 · US
Lov-D acyltransferase mediated acylation
US10428360B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10428360-B2 |
| Application number | US-201815887646-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 2, 2018 |
| Priority date | Sep 30, 2009 |
| Publication date | Oct 1, 2019 |
| Grant date | Oct 1, 2019 |
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Abstract
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Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of making simvastatin, comprising contacting monacolin J substrate with a LovD polypeptide having acyltransferase activity, wherein said LovD polypeptide is a recombinant variant comprising SEQ ID NO: 20, in the presence of an alpha-dimethylbutyryl thioester co-substrate, under conditions such that said monacolin J substrate is converted to simvastatin. 2. The method of claim 1 , wherein said monacolin J substrate is a monacolin J hydroxyl acid salt. 3. The method of claim 1 , said monacolin J substrate is a monacolin J sodium salt. 4. The method of claim 1 , wherein said monacolin J substrate is monacolin J lactone. 5. The method of claim 1 , said monacolin J substrate is a monacolin J ammonium salt. 6. The method of claim 1 , wherein an agent for precipitating simvastatin is added. 7. The method of claim 6 , wherein said agent is ammonium hydroxide. 8. The method of claim 1 , wherein said method is carried out in an aqueous medium at a pH in the range of pH 8 to pH 9.5. 9. The method of claim 1 , wherein said method is carried out in an aqueous buffer having an initial pH of 9. 10. The method of claim 1 , wherein said method further comprises a thiol scavenging agent. 11. The method of claim 10 , wherein said thiol scavenging agent comprises activated charcoal.
Assignees
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Classifications
Carboxylic acid esters · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
Acyltransferases (2.3) · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
using catalysts, e.g. selective catalysts · CPC title
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- What does patent US10428360B2 cover?
- Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P17/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 01 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).