Adipose tissue centrifuge and method of use

Patent metadata

Field	Value
Publication number	US-12018244-B2
Application number	US-202318094446-A
Country	US
Kind code	B2
Filing date	Jan 9, 2023
Priority date	Jan 31, 2014
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

First claim

What is claimed is:

1. A method for separating and collecting a component from a tissue material comprising the steps of:
a. introducing a tissue material into a chamber of a centrifuge, said chamber having a central longitudinal axis about which said chamber is arranged to be rotated and said chamber comprising a sidewall with an inner surface that increases in distance from the central longitudinal axis along a length of the sidewall, an annular screen having an inner surface located at a first radial distance from said central longitudinal axis, and a first port, the first port being located in said sidewall at a second radial distance from said central longitudinal axis, wherein the second radial distance is greater than the first radial distance and the second radial distance is at or proximate a greatest radial distance from the central longitudinal axis within the chamber,
b. rotating said chamber about the central longitudinal axis, thereby creating a centrifugal force on the tissue material sufficient to pass at least a substantial portion of the tissue material through said annular screen as a result of the centrifugal force and thereby morselizing the substantial portion of the tissue material, and
c. collecting at least a portion of a highest specific gravity constituent of the tissue material that has passed through the first port.

2. The method of claim 1 , wherein the tissue material is adipose tissue and the highest specific gravity component comprises stem cells.

3. The method of claim 1 , wherein the first port is selectively openable.

4. The method of claim 1 , wherein the portion of the highest specific gravity constituent of the tissue material that has passed through the first port is collected in a trap.

5. The method of claim 4 , wherein the chamber of the centrifuge is surrounded by an outer housing, the outer housing comprising an access opening therein, wherein the access opening provides access to the trap.

6. The method of claim 5 , further comprising the step of:
d. extracting at least the portion of the highest specific gravity constituent of the tissue material from the trap via the access opening, wherein the portion of the tissue material so extracted comprises stem cells.

7. The method of claim 6
wherein the step of extracting comprises inserting a syringe into the access opening.

8. The method of claim 1 , wherein the chamber comprises a base and the annular screen projects away from the base.

9. The method of claim 4 , wherein the chamber comprises a base and the annular screen projects away from the base, wherein the chamber further comprises a wedge present in the base, the wedge comprising an outer surface that is angled relative to the central longitudinal axis, and wherein an outer surface of the wedge and the inner surface of the sidewall define the trap.

10. The method of claim 9 , wherein the wedge comprises an angled inner surface, and wherein the chamber comprises a dished area in the base, the dished area at least partially defined by the angled inner surface of the wedge.

11. The method of claim 10 , wherein upon cessation of the rotation of the chamber, one or more constituent layers settle into the dished area of the chamber and do not enter the trap.

12. The method of claim 1 , wherein the chamber further comprises a secondary annular screen, the secondary annular screen being located at a third radial distance from the central longitudinal axis, wherein the third radial distance is greater than the first radial distance and less than the second radial distance, wherein the secondary annular screen is configured to capture fibrous material while allowing non-fibrous material and liquid to pass through.

13. The method of claim 1 , wherein the centrifuge further comprises a roller arranged to urge at least a portion of the tissue material through the annular screen and away from the central longitudinal axis and towards the sidewall.

14. The method of claim 1 , wherein the centrifuge further comprises a roller arranged to roll against and around the inner surface of the annular screen and urge at least a portion of the tissue material through the annular screen and away from the central longitudinal axis and towards the sidewall.

15. The method of claim 12 , wherein the centrifuge further comprises a roller arranged to urge at least a portion of the tissue material through the annular screen and away from the central longitudinal axis and towards the sidewall.

16. The method of claim 12 , wherein the centrifuge further comprises a roller arranged to roll against and around the inner surface of the annular screen and urge at least a portion of the tissue material through the annular screen and away from the central longitudinal axis and towards the sidewall.

17. The method of claim 1 , wherein the tissue material is fibrous.

18. The method of claim 1 , wherein the second radial distance is the greatest radial distance from the central longitudinal axis within the chamber.

19. A method for separating a cellular component from a fibrous tissue material comprising the steps of:
a. introducing a fibrous tissue material into a chamber of a centrifuge, said chamber having a central longitudinal axis about which said chamber is arranged to be rotated and said chamber comprising a sidewall with an inner surface that increases in distance from the central longitudinal axis along a length of the sidewall, a first port located in the sidewall and in fluid communication with the interior of the chamber, the first port located at the greatest distance from the central longitudinal axis within the chamber, and a screen located between the first port and the central longitudinal axis,
b. rotating said chamber about the central longitudinal axis, thereby creating a centrifugal force on the fibrous tissue material,
c. causing at least a substantial portion of the fibrous tissue material to be morselized by and pass through said screen as a result of the centrifugal force, and
d. directing at least a portion of a highest specific gravity constituent of the fibrous tissue material to the first port via contact with the sidewall, wherein the highest specific gravity constituent comprises a cellular component.

20. The method of claim 19 , wherein step c is supplemented by a roller arranged to urge at least a portion of the fibrous tissue material through the screen and away from the central longitudinal axis and towards the sidewall.

Assignees

• Dsm Ip Assets Bv

Inventors

• Nash John E
• Fisher William T
• Bradica Gino

Classifications

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B04B7/12

  Inserts, e.g. armouring plates · CPC title

• B04B3/00 Primary

  Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering · CPC title

• C12N5/0667

  Adipose-derived stem cells [ADSC]; Adipose stromal stem cells · CPC title

• C12N2527/00

  Culture process characterised by the use of mechanical forces, e.g. strain, vibration · CPC title