Liquid separation from adipose tissue

Patent metadata

Field	Value
Publication number	US-9604159-B2
Application number	US-201314033944-A
Country	US
Kind code	B2
Filing date	Sep 23, 2013
Priority date	Jun 16, 2009
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

Extracting or removing at least a portion of liquid phase from a whole sample using a centrifugal force is disclosed. Centrifugal forces are used to apply pressure to a whole sample and drive a liquid phase through a passage region that can be perforated and/or porous and maintain a drier portion within a separation container. The whole sample can be dried, which includes a remaining sample where excess or a selected amount of liquid is removed. Direct access to the separation container or area can then be made to provide for an efficient withdrawal of the drier material from the separation container.

First claim

What is claimed is:

1. A method of reducing fluid in a whole adipose tissue sample, comprising:
obtaining a whole adipose tissue sample from a patient;
placing the obtained whole adipose tissue sample into a first separation region of a separation container having a collection basin surrounding at least a portion of the first separation region, wherein the first separation region is centered on a central axis of the separation container;
surrounding the separation container with an exterior container;
spinning the first separation region within the exterior container about the central axis of the separation container to place a centrifugal force on the obtained whole adipose tissue sample placed in the separation container;
forcing at least a portion of the whole adipose tissue sample radially outward from the central axis against an inner wall surface near a perforated wall portion, wherein the inner wall surface extends radially around the central axis;
moving a volume of a liquid phase through the perforated wall portion of the separation container into the collection basin surrounding at least a portion of the first separation region while spinning the separation container;
containing adipose particles of the whole adipose tissue sample within the first separation region;
collecting adipose particles in a withdrawal area of the first separation region after the volume of the liquid phase has been removed; and
withdrawing the adipose particles while maintaining the separation container within the exterior container.

2. The method of claim 1 , further comprising:
connecting the separation container to a motor; and
operating the motor to spin the separation container; wherein the motor is operated to spin the separation container at least at about 2000 rotations per minute for at least about 30 seconds.

3. The method of claim 2 , wherein operating the motor to spin the separation container induces the centrifugal force on the whole adipose tissue sample placed in the separation container;
wherein moving a volume of the liquid phase includes forcing a liquid portion of the whole adipose tissue sample through the perforated wall portion having bores with a first passage cross-section appropriate to contain the adipose particles within the first separation region.

4. The method of claim 3 , further comprising:
placing a material defining a second passage cross-section over the perforated portion;
wherein the second passage cross-section is less than the first passage cross-section.

5. The method of claim 4 , further comprising:
wherein the liquid phase is operable to move through at least the perforated portion, the material, and through a portion of the whole adipose tissue sample.

6. The method of claim 5 , further comprising:
maintaining the separation container substantially sterile while placing the obtained whole adipose tissue sample into the first separation region and withdrawing the drier adipose particles.

7. The method of claim 1 , wherein moving the volume of the liquid phase through the perforated wall portion of the separation container into the collection basin includes moving at least one of about 30%, about 50%, about 70%, about 80%, or about 90% of the liquid phase from the whole adipose tissue sample.

8. The method of claim 1 , wherein withdrawing the adipose particles includes accessing the separation container through an access port through the exterior container to allow extraction of a drier portion of the whole adipose sample from the separation container.

9. The method of claim 8 , wherein withdrawing the adipose particles further includes withdrawing the drier portion of the whole adipose sample from the separation container through a tube extending from the access port.

10. The method of claim 1 , further comprising:
moving the volume of the liquid phase through a porous material covering at least a first plurality of throughbores of a plurality of throughbores that are formed through the perforated wall portion of the separation container.

11. A method of reducing fluid in a whole adipose tissue sample, comprising:
placing a whole adipose tissue sample into a first separation region of a separation container having a collection basin surrounding at least a portion of the first separation region, wherein the first separation region is centered on a central axis of the separation container;
forming a drier volume of adipose tissue by rotating the first separation region about the central axis to move a volume of a liquid phase of the whole adipose tissue sample radially outward from the central axis through at least one bore of a perforated wall portion of the separation container into the collection basin surrounding at least a portion of the first separation region; and
withdrawing at least a portion of the drier volume of adipose tissue from the first separation region of the separation container.

12. The method of claim 11 , wherein withdrawing at least the portion of the drier volume of adipose tissue from the first separation region of the separation container occurs while the separation container is maintained within an exterior container.

13. The method of claim 11 , wherein forming the drier volume of adipose tissue further includes:
forcing at least a portion of the whole adipose tissue sample against an inner wa surface near the perforated wall portion defining the at least one bore.

14. The method of claim 13 , wherein forcing at least the portion of the whole adipose tissue sample against an inner wall surface includes spinning the separation container around the central axis of the separation container to place a centrifugal force on the whole adipose tissue sample placed in the separation container.

15. The method of claim 14 , further comprising:
ceasing spinning of the separation container; and
containing the drier volume of adipose tissue within the first separation region.

16. The method of claim 15 , wherein containing the drier volume of adipose tissue within the first separation region includes collecting adipose particles in a withdrawal area of the first separation region after the volume of the liquid phase has been removed into the collection basin.

17. The method of claim 11 , wherein forming the drier volume of adipose tissue further includes moving the volume of the liquid phase of the whole adipose tissue sample through a porous material covering the at least one bore.

18. The method of claim 11 , further comprising:
maintaining the separation container within an exterior container during all of placing the whole adipose tissue sample into the first separation region of the separation container, forming the drier volume of adipose tissue, and withdrawing at least the portion of the drier volume of adipose tissue from the separation container.

19. A method of reducing fluid in a whole adipose tissue sample, comprising:
placing a whole adipose tissue sample into a first separation region of a separation container having a collection basin surrounding at least a portion of the first separation region;
operating a system to spin the separation container to form a drier volume of adipose tissue by moving a volume of a liquid phase of the whole adipose tissue sample through a porous material and at least one bore of a perforated wall portion of the separation container into the collection basin surrounding at least a portion of the first separation region, wherein the porous material is placed over the at least one bore; and
withdrawing at least a portion

Assignees

• Biomet Biologics Llc

Inventors

• Leach Michael D
• Chavarria Jason

Classifications

• B01D17/0217 Primary

  by centrifugal force · CPC title

• B04B3/00

  Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering · CPC title

• A61M2202/08

  Lipoids · CPC title

• A61B2017/00969

  used for transplantation · CPC title

• B04B5/0442

  with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation · CPC title