Light irradiation apparatus

Patent metadata

Field	Value
Publication number	US-12000771-B2
Application number	US-202017630142-A
Country	US
Kind code	B2
Filing date	Jul 27, 2020
Priority date	Jul 25, 2019
Publication date	Jun 4, 2024
Grant date	Jun 4, 2024

Abstract

A light irradiation device including an injection unit to inject a sample, and a light source to apply light to the sample to identify an abnormal cell in the sample, the light source including a substrate and a light emitter including a light emitting diode, in which the light emitted is to cut a genetic material in the sample into sections of different sizes, and to deform the genetic material to different degrees, such that a determination of an abnormality of the sample is based on the degree of deformation, and an irradiation amount or intensity of the light is at an intensity in which a cytotoxicity value of the sample is greater than or equal to a predetermined value, and a ratio of a degree of deformation of the genetic material of the normal sample to that of the abnormal sample is set to be a minimum value.

First claim

What is claimed is:

1. A light irradiation device comprising:
an injection unit configured to inject a sample; and
a light source configured to apply light to the sample to identify an abnormal cell in the sample, the light source including:
a substrate; and
a light emitter disposed on the substrate and comprising a light emitting diode,

wherein the light emitted from the light emitter is configured to cut a genetic material in the sample into sections of different sizes depending on a normal sample or an abnormal sample, and to deform the genetic material to different degrees, such that a determination of an abnormality of the sample is based on the degree of deformation of the genetic material, and
wherein an irradiation amount or an irradiation intensity of the light emitted from the light emitter is configured to be provided at an intensity in which a cytotoxicity value of the sample is greater than or equal to a predetermined value, and in which a ratio of a degree of deformation of the genetic material of the normal sample to a degree of deformation of the genetic material of the abnormal sample is set to be a minimum value outside an error range.

2. The light irradiation device of claim 1 , wherein the light emitted from the light emitter is in a wavelength band that generates reactive oxygen species, such that the light transmitted into the sample stimulates a photosensitive material in the sample.

3. The light irradiation device of claim 1 , further comprising:
a reaction unit configured to inject a dyeing material into the sample to cause reaction between the dyeing material and the sample; and
an analysis unit configured to analyze the sample.

4. The light irradiation device of claim 3 , further comprising a pre-processing unit configured to process the sample before providing the sample to the reaction unit.

5. The light irradiation device of claim 4 , wherein the sample comprises blood.

6. The light irradiation device of claim 5 , wherein the sample comprises leukocyte, and the pre-processing unit is configured to separate the leukocyte from whole blood.

7. The light irradiation device of claim 3 , wherein the analysis unit is configured to be implemented in a Comet assay.

8. The light irradiation device of claim 7 , wherein the analysis unit includes:
a data providing unit configured to provide a table quantifying a degree of damage of the genetic material according to a length of a tail in an electrophoresis result;
a comparison unit configured to compare lengths of tails of a normal cell and an abnormal cell with the length of the tail stored in the data providing unit; and
a determination unit configured to determine whether cancer occurs based on the comparison result of the comparison unit.

9. The light irradiation device of claim 8 , wherein the data providing unit has a table corresponding to either a normal or an abnormal state according to the length of the tail.

10. The light irradiation device of claim 1 , wherein the light emitted from the light emitter includes at least a portion of light in a blue wavelength band to an ultraviolet wavelength band.

11. The light irradiation device of claim 10 , wherein the irradiation amount of the light is configured to be set within a limit in which cell viability of the normal sample is 75% or more.

12. The light irradiation device of claim 10 , wherein the irradiation amount of the light is configured to be about 5 J/cm 2 or less, and the light is configured to be irradiated with an irradiation time of about 15 minutes or less.

13. The light irradiation device of claim 10 , wherein the light is configured to be irradiated with a quantity of light of about 1.5 mW/cm 2 to about 100 mW/cm 2 .

14. The light irradiation device of claim 1 , wherein the light corresponds to at least one of light in UVA, UVB, and UVC wavelength bands and light in the blue wavelength band.

15. The light irradiation device of claim 1 , wherein the light source includes at least two light emitters configured to emit light having different wavelengths.

16. A light irradiation device comprising:
an injection unit configured to inject a sample; and
a light source configured to apply light to the sample, wherein the light source includes:
a light emitter configured to emit light to the sample to identify an abnormal cell in the sample; and
a substrate on which the light emitter is disposed to dissipate heat generated from the light emitter,

wherein the light emitted from the light emitter is configured to cut a DNA or RNA in the sample into sections of different sizes depending on a normal sample or an abnormal sample, and to deform the DNA or RNA to different degrees, such that a determination of the abnormality of the sample is based on the degree of deformation of the DNA or RNA, and
wherein an irradiation amount or irradiation intensity of the light is configured to be set within a limit in which cell viability of the normal sample is 75% or more.

17. The light irradiation device of claim 16 , wherein the light emitted from the light emitter includes at least a portion of light in a blue wavelength band to an ultraviolet wavelength band.

18. The light irradiation device of the claim 16 , wherein the irradiation amount of the light is configured to be about 5 J/cm 2 or less, and the light is configured to be irradiated with an irradiation time of about 15 minutes or less.

19. The light irradiation device of claim 16 , wherein the light is configured to be irradiated with a quantity of light of about 1.5 mW/cm 2 to about 100 mW/cm 2 .

20. The light irradiation device of claim 16 , wherein the sample comprises blood.

Assignees

• Seoul Viosys Co Ltd

Inventors

• Bae Hee Ho
• Yoon Yeong Min
• Lee A Young

Classifications

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G01N2021/0112

  Apparatus in one mechanical, optical or electronic block · CPC title

• G01N27/447

  using electrophoresis · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title