Apparatus for detecting abnormal cells using fluorescent image analysis and method for the same

Patent metadata

Field	Value
Publication number	US-11287380-B2
Application number	US-201816204443-A
Country	US
Kind code	B2
Filing date	Nov 29, 2018
Priority date	Nov 30, 2017
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

An image analysis apparatus and an image analysis method capable of appropriately analyzing whether an analysis target cell is an abnormal cell even when a subject has a chromosomal abnormality are provided. An image analysis apparatus 10 includes light sources 121 and 122 configured to irradiate light onto a sample 21 whose target portion is labeled, an imaging unit 154 for capturing light generated from the sample 21 by irradiation with light, and a processing unit 11 that processes the image captured by the imaging unit 154. The processing unit 11 acquires information on the chromosomal abnormality and analyzes the image based on the information on the acquired chromosome abnormality.

First claim

What is claimed is:

1. An image analysis apparatus comprising:
a light source configured to irradiate light on a sample of a subject, the sample containing a cell having labeled target sites in different colors;
an imaging unit configured to:
capture light from the labeled target site irradiated by the light source and generate an image of a cell comprising at least one bright spot of a plurality of bright spots corresponding to the labeled target sites; and
a processing unit configured to process the image generated by the imaging unit by counting the bright spots for each of the colors in the image, comparing a combination of numbers of bright spots in different colors with a reference combination that is selected based on information of the subject, and determining that the cell in the image is an abnormal cell when the combination of numbers of bright spots in the image matches with the selected reference combination, wherein the reference combination includes a number of a bright spot of a first color, a number of a bright spot of a second color, and a number of a bright spot in which the bright spot of the first color overlaps with the bright spot of the second color,
wherein the processing unit acquires the information of the subject and selects one of a plurality of combinations as the reference combination, the plurality of combinations including:
a first combination corresponding to the information of the subject indicating that the subject does not have a predetermined chromosomal abnormality; and
a second combination corresponding to the information of the subject indicating that the subject has the predetermined chromosomal abnormality.

2. The image analysis apparatus according to claim 1 , wherein the processing unit analyzes the cell based on a combination of the bright spots corresponding to the plurality of the target sites in the image and the acquired information of the subject, wherein the cell has a plurality of the target sites.

3. The image analysis apparatus according to claim 1 , wherein the information of the subject comprises a past examination result of the subject.

4. The image analysis apparatus according to claim 1 , wherein the information of the subject comprises a disease name of the subject.

5. The image analysis apparatus according to claim 1 , wherein the information of the subject comprises information indicating whether a performed transplantation caused a change in a chromosome of the subject.

6. The image analysis apparatus according to claim 1 , wherein the information of the subject comprises chromosomal abnormality.

7. The image analysis apparatus according to claim 2 , wherein the combination of the bright spots comprises a combination of the color and the number of the bright spots.

8. The image analyzing apparatus according to claim 2 further comprising:
a storage unit that stores the plurality of combinations,
wherein the processing unit selects the reference combination corresponding to the information of the subject from the plurality of combinations, and determines an analysis result of the cell based on the selected reference combination.

9. The image analysis apparatus of claim 8 , wherein
the storage unit stores combinations of the bright spots for each measurement item; and
the processing unit selects the reference combination corresponding to the measurement item and the information of the subject, and determines an analysis result of the cell based on the selected reference combination.

10. The image analysis apparatus according to claim 1 , further comprising:
a display unit;
wherein the processing unit causes the display unit to display a subject information receiving screen for receiving the information of the subject, and acquires the information of the subject via the subject information receiving screen.

11. The image analysis apparatus according to claim 1 , further comprising:
a communication unit for communicating with an external computer that stores the information of the subject;
wherein the processing unit acquires the information of the subject from the external computer via the communication unit.

12. The image analysis apparatus according to claim 1 , wherein
the processing unit analyzes the cell and determines whether the cell is abnormal.

13. The image analysis apparatus according to claim 12 , wherein
the processing unit determines whether the cell is abnormal based on whether a combination of bright spots corresponding to the labeled target site in the image matches a combination of bright spots corresponding to abnormal or normal cases corresponding to the information of the subject.

14. The image analysis apparatus according to claim 1 , further comprising:
a display unit;
wherein the processing unit calculates a number or a ratio of abnormal cells in the sample and causes a calculation result to be displayed on the display unit.

15. The image analysis apparatus according to claim 6 , further comprising:
a display unit;
wherein the processing unit
analyzes each of a plurality of cells included in the sample;
calculates a number or a ratio of the cells that do not match the combination of the bright spots in both abnormal and normal cases; and
displays a reanalysis acceptance screen for accepting an instruction to reanalyze an analyzed cell; and
the analyzed cell is reanalyzed based on the information of the subject in response to an acceptance of the instruction for reanalysis.

16. An image analysis method comprising:
irradiating light on a sample of a subject, the sample containing a cell having labeled target sites in different colors;
capturing light from the irradiated labeled target site;
generating an image of a cell comprising at least one bright spot of a plurality of bright spots corresponding to the labeled target sites;
processing the generated image by counting the bright spots for each of the colors in the image, comparing a combination of numbers of bright spots in different colors with a reference combination that is selected based on information of the subject, and determining that the cell in the image is an abnormal cell when the combination of numbers of bright spots in the image matches with the selected reference combination, wherein the reference combination includes a number of a bright spot of a first color, a number of a bright spot of a second color, and a number of a bright spot in which the bright spot of the first color overlaps with the bright spot of the second color; and
acquiring the information of the subject and selecting one of the plurality of combinations as the reference information, the plurality of combinations including:
a first combination corresponding to the information of the subject indicating that the subject does not have a predetermined chromosomal abnormality; and
a second combination corresponding to the information of the subject indicating that the subject has the predetermined chromosomal abnormality.

Assignees

• Sysmex Corp

Inventors

• Akasaka Yuki

Classifications

• G01N2015/144

  Imaging characterised by its optical setup · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• G01N2021/6441

  with two or more labels · CPC title

• G01N2021/6419

  Excitation at two or more wavelengths · CPC title

• G06T2207/30004

  Biomedical image processing · CPC title