Wet milling process

Patent metadata

Field	Value
Publication number	US-11987649-B2
Application number	US-202318299638-A
Country	US
Kind code	B2
Filing date	Apr 12, 2023
Priority date	Nov 26, 2015
Publication date	May 21, 2024
Grant date	May 21, 2024

Abstract

The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

First claim

What is claimed is:

1. A process for treating crop kernels, comprising:
a) soaking kernels in water to produce soaked kernels;
b) grinding the soaked kernels to form ground kernels;
c) separating the germ from the ground kernels to produce a slurry comprising fiber, starch and protein; and
d) treating the slurry in a fiber washing step to separate fiber from the starch and protein in the presence of an effective amount of a polypeptide having arabinofuranosidase activity, wherein the polypeptide having arabinofuranosidase activity comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 27.

2. The process of claim 1 , wherein step d) further comprises treating the slurry in the presence of a protease.

3. The process of claim 1 , wherein step d) further comprises treating the slurry in the presence of a cellulolytic enzyme.

4. The process of claim 1 , wherein step d) further comprises treating the slurry in the presence of an enzyme selected from the group consisting of an endoglucanase, a xylanase, a cellobiohydrolase I, a cellobiohydrolase II, a GH61, or a combination thereof.

5. The process of claim 1 , wherein the kernels are soaked in water for about 2-10 hours.

6. The process of claim 1 , wherein the soaking is carried out at a temperature between about 40° C. and about 60° C.

7. The process of claim 1 , wherein the soaking is carried out at an acidic pH.

8. The process of claim 1 , wherein the soaking is performed in the presence of between 0.01-1% SO 2 and/or NaHSO 3 .

9. The process of claim 1 , wherein the crop kernels are from corn (maize), rice, barley, sorghum bean, or fruit hulls, or wheat.

10. The process of claim 1 , wherein step d) further comprises treating the slurry in the presence of a GH10 xylanase or a GH11 xylanase.

11. The process of claim 10 , wherein the GH10 xylanase is a Talaromyces, Trichophaea , or Aspergillus GH10 xylanase.

12. The process of claim 1 , further treating the slurry in the presence of a GH10 xylanase, an endoglucanase, a cellobiohydrolase I, a cellobiohydrolase II, and a GH61.

13. The process of claim 1 , further comprising separating the starch from the protein.

14. The process of claim 13 , further comprising washing the starch to produce a pure starch.

15. The process of claim 14 , further comprising converting the starch to syrup or alcohol.

16. The process of claim 1 , wherein the polypeptide having arabinofuranosidase activity comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 27.

17. The process of claim 1 , wherein the polypeptide having arabinofuranosidase activity comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 27.

18. The process of claim 1 , wherein the polypeptide having arabinofuranosidase activity comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 27.

19. The process of claim 1 , wherein the polypeptide having arabinofuranosidase activity comprises an amino acid sequence having at least 99% sequence identity to SEQ ID NO: 27.

20. The process of claim 1 , wherein the polypeptide having arabinofuranosidase activity comprises the amino acid sequence of SEQ ID NO: 27.

Assignees

• Novozymes As

Inventors

• Lavigne James
• Vidal Jr Bernardo
• Gibbons Thomas Patrick
• Soong Chee-Leong
• Deinhammer Randall Scott
• Long Zhen
• Cao Yi
• Akerman Michael John
• Shen Xinyu
• Zhang Yu
• Scott Brian R

Classifications

• C08B30/044 Primary

  from corn or maize · CPC title

• C08B30/02

  Preparatory treatment, e.g. crushing of raw materials {or steeping process (machines for preliminary washing A23N)} · CPC title

• C08H99/00 Primary

  Subject matter not provided for in other groups of this subclass {, e.g. flours, kernels} · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title