Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity

Patent metadata

Field	Value
Publication number	US-10711259-B2
Application number	US-201515528910-A
Country	US
Kind code	B2
Filing date	Dec 18, 2015
Priority date	Dec 19, 2014
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity for use in e.g. animal feed. Polypeptides having arabinofuranosidase activity, polypeptides having xylanase activity and polynucleotides encoding the polypeptides. Nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptide.

First claim

What is claimed is:

1. A composition comprising
a GH10 polypeptide having xylanase activity at least 85% sequence identity to the polypeptide of SEQ ID NO: 70, and
a GH62 polypeptide having arabinofuranosidase activity and at least 85% sequence identity to the polypeptide of SEQ ID NO: 12,

wherein:
(a) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2.0% xylose from defatted destarched maize (DFDSM); and
(b) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2 times more xylose from DFDSM than the GH10 polypeptide in the absence of the GH62 polypeptide;

wherein (a) and (b) are performed under the reaction conditions: (i) 25 mg GH10 polypeptide per kg DFDSM, (ii) 12.5 mg GH62 polypeptide per kg DFDSM, and (iii) incubation at 40° C., pH 5 for 4 hours.

2. The composition of claim 1 , wherein
the GH10 polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 70, and
the GH62 polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 12.

3. The composition of claim 1 , wherein
the GH10 polypeptide has at least 95% sequence identity to the polypeptide of SEQ ID NO: 70, and
the GH62 polypeptide has at least 95% sequence identity to the polypeptide of SEQ ID NO: 12.

4. The composition of claim 1 , wherein
GH10 polypeptide has at least 346 amino acid residues and has xylanase activity, and
the GH62 polypeptide has at least 273 amino acid residues and has arabinofuranosidase activity.

5. The composition of claim 1 , wherein
the GH10 polypeptide has at least 85% sequence identity to the polypeptide of SEQ ID NO: 70 with one or more amino acid substitutions, deletions and/or insertions to the polypeptide of SEQ ID NO: 70, and
the GH62 polypeptide has at least 85% sequence identity to the polypeptide of SEQ ID NO: 12 with one or more amino acid substitutions, deletions and/or insertions to the polypeptide of SEQ ID NO: 12.

6. The composition of claim 1 , wherein
the GH10 polypeptide comprises SEQ ID NO: 70, and
the GH62 polypeptide comprises SEQ ID NO: 12.

7. An animal feed additive comprising the composition of claim 1 and one or more components selected from the list consisting of:
one or more vitamins;
one or more minerals;
one or more amino acids; and
one or more other feed ingredients.

8. An animal feed comprising the composition of claim 1 and plant-based material from the sub-family Panicoideae.

9. A method of improving one or more performance parameters of an animal comprising administering to one or more animals the composition of claim 1 .

10. A method of solubilising xylose from plant based material, comprising treating plant based material from the sub-family Panicoideae with the composition of claim 1 .

11. A method of releasing starch from plant based material, comprising treating plant based material from the sub-family Panicoideae with the composition of claim 1 .

12. A method for improving the nutritional value of an animal feed, comprising adding to the feed the composition of claim 1 .

13. A method of preparing an animal feed, comprising mixing the composition of claim 1 with plant based material from the sub-family Panicoideae.

Assignees

• Novozymes As

Inventors

• Peng Wei
• Pedersen Ninfa Rangel
• Pettersson Dan
• Ekloff Jens Magnus
• Nymand-Grarup Soren
• Palmen Lorena G
• Monrad Rune Nygaard
• Spodsberg Nikolaj
• Stringer Mary Ann
• Blom Charlotte
• Kiemer Lars
• Krogh Kristian Bertel Romer M
• Salomon Jesper

Classifications

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12Y302/01008

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C07K2319/55

  containing a fusion with a toxin, e.g. diphteria toxin · CPC title

• C07K2319/21

  containing a His-tag · CPC title