Insecticidal proteins and methods for their use

Patent metadata

Field	Value
Publication number	US-11959090-B2
Application number	US-202117242370-A
Country	US
Kind code	B2
Filing date	Apr 28, 2021
Priority date	Nov 1, 2016
Publication date	Apr 16, 2024
Grant date	Apr 16, 2024

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed:

1. An insecticidal polypeptide comprising an amino acid sequence having at least 97% sequence identity compared to the amino acid sequence of SEQ ID NO: 70, wherein the insecticidal polypeptide is joined to a heterologous signal sequence, a transit sequence, or a histidine tag, or fused to a protein of interest either directly or through a linker segment.

2. An insecticidal composition comprising the insecticidal polypeptide of claim 1 .

3. A recombinant polynucleotide operably linked to a heterologous regulatory element, wherein the polynucleotide encodes an insecticidal polypeptide comprising an amino acid sequence having at least 97% sequence identity compared to the amino acid sequence of SEQ ID NO: 70.

4. The recombinant polynucleotide of claim 3 , wherein the recombinant polynucleotide is the polynucleotide of SEQ ID NO: 30.

5. A DNA construct comprising the recombinant polynucleotide of claim 3 .

6. A transgenic plant or plant cell comprising the DNA construct of claim 5 .

7. A method of inhibiting growth or killing a western corn rootworm insect pest, said method comprising contacting the insect pest with an insecticidally-effective amount of the polypeptide of claim 1 .

8. A method of controlling a western corn rootworm insect infestation in a transgenic plant and providing insect resistance management, said method comprising transforming the polynucleotide of claim 3 into a plant.

9. The method of claim 7 , wherein the insect pest or insect pest population is resistant to a Bt toxin.

Assignees

• Pioneer Hi Bred Int

Inventors

• Altier Daniel J
• Barry Jennifer Kara
• Gerber Ryan Michael
• Gruver Steven D
• Liu Lu
• Schellenberger Ute
• Wei Jun-Zhi
• Xie Weiping
• Yalpani Nasser
• Zhu Genhai

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• C07K14/195

  from bacteria · CPC title

• C07K14/21 Primary

  from Pseudomonadaceae (F) · CPC title

• C07K14/265

  Enterobacter (G) · CPC title