Insecticidal proteins and methods for their use

Patent metadata

Field	Value
Publication number	US-11021716-B2
Application number	US-201716345764-A
Country	US
Kind code	B2
Filing date	Sep 14, 2017
Priority date	Nov 1, 2016
Publication date	Jun 1, 2021
Grant date	Jun 1, 2021

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed:

1. A DNA construct comprising a polynucleotide encoding an insecticidal polypeptide, wherein the insecticidal polypeptide comprises an amino acid sequence having greater than 95% sequence identity compared to the amino acid sequence of SEQ ID NO: 43, and wherein the polynucleotide is operably linked to a heterologous regulatory element.

2. The DNA construct of claim 1 , wherein the recombinant polynucleotide comprises
the polynucleotide of SEQ ID NO: 1.

3. A transgenic plant or plant cell comprising the DNA construct of claim 1 .

4. A method of controlling a Lepidoptera and/or Coleoptera insect infestation in a transgenic plant and providing insect resistance management, said method comprising transforming the DNA construct of claim 1 into a plant.

Assignees

• Pioneer Hi Bred Int

Inventors

• Altier Daniel J
• Barry Jennifer Kara
• Gerber Ryan Michael
• Gruver Steven D
• Liu Lu
• Schellenberger Ute
• Wei Jun-Zhi
• Xie Weiping
• Yalpani Nasser
• Zhu Genhai

Classifications

• C07K14/195

  from bacteria · CPC title

• C07K14/21 Primary

  from Pseudomonadaceae (F) · CPC title

• C07K14/32

  from Bacillus (G) · CPC title

• C07K14/33

  from Clostridium (G) · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title