Method of cultivating LC-PUFA containing transgenic brassica plants

Patent metadata

Field	Value
Publication number	US-11957098-B2
Application number	US-201917046989-A
Country	US
Kind code	B2
Filing date	Apr 11, 2019
Priority date	Apr 13, 2018
Publication date	Apr 16, 2024
Grant date	Apr 16, 2024

Abstract

Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13° C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.

First claim

What is claimed is:

1. A method of increasing the proportion of long-chain omega-3 fatty acids in seed oil produced by a plurality of transgenic Brassica napus oilseed plants, comprising growing said transgenic Brassica napus oilseed plants at a daytime temperature of about 25° C., and a nighttime temperature of about 12° C., wherein said growing is for a period of seed maturation for said transgenic Brassica napus oilseed plants, wherein said period of seed maturation comprises BBCH-scale growth stage 6, BBCH-scale growth stage 7 and BBCH-scale growth stage 8 of Brassica napus oilseed plant growth stages, wherein said transgenic Brassica napus oilseed plants have been transgenically modified to produce said seed oil comprising at least one of EPA, DHA, and DPA, and wherein the proportion of long-chain omega-3 fatty acids in said seed oil produced by said transgenic Brassica napus oilseed plants is increased corresponding to controls of Brassica napus oilseed plants.

2. The method of claim 1 , wherein said transgenic Brassica napus oilseed plants are grown in a growth chamber, a green house, a partially-enclosed outdoors environment, or an open field.

3. The method of claim 1 , wherein said transgenic Brassica napus oilseed plants are grown in a field.

4. The method of claim 1 , wherein said seed oil is at least 5 wt % EPA.

5. The method of claim 1 , wherein said seed oil is at least 1 wt % DPA.

6. The method of claim 1 , wherein said seed oil is at least 0.2 wt % DHA.

7. The method of claim 1 , wherein said seed oil is at least 5.2 wt % a mixture of EPA and DHA.

8. The method of claim 1 , wherein said seed oil is at least 6 wt % long chain omega-3 fatty acids.

9. The method of claim 1 , wherein said seed oil comprises between 5 wt % EPA and 25 wt % EPA.

10. The method of claim 1 , wherein said seed oil comprises between 1 wt % DPA and 20 wt % DPA.

11. The method of claim 1 , wherein said seed oil comprises between 0.2 wt % DHA and 30 wt % DHA.

12. The method of claim 1 , wherein said seed oil comprises between 1 wt % and 20 wt % of a mixture of EPA and DHA.

13. The method of claim 1 , wherein said seed oil comprises between 1 wt % long chain omega-3 fatty acids and 30 wt % long chain omega-3 fatty acids.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Deng Xinmin
• Gray Kristin
• Hasan Jakir
• Horton Keith

Classifications

• A01H1/105 Primary

  involving altered sterol composition · CPC title

• A01H5/10

  Seeds · CPC title

• A01H6/202

  Brassica napus [canola] · CPC title

• A01H3/02 Primary

  by controlling duration, wavelength, intensity, or periodicity of illumination · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title