Drought and heat tolerance in plants

Patent metadata

Field	Value
Publication number	US-11946060-B2
Application number	US-202016790485-A
Country	US
Kind code	B2
Filing date	Feb 13, 2020
Priority date	Feb 15, 2008
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

Methods and materials for modulating heat and/or drought tolerance in plants are disclosed. For example, nucleic acids encoding heat and/or drought-tolerance polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased heat and/or drought tolerance and plant products produced from plants having increased heat and/or drought tolerance.

First claim

What is claimed is:

1. A method of producing a plant, said method comprising
growing a plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence comprising a polynucleotide sequence having 90 percent or greater identity to the polynucleotide sequence of SEQ ID NO:363, or wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequences having 90 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:364,
producing a plant from said plant cell,
exposing the plant produced from said plant cell to a heat shock comprising 42° C. for 5 hours,
selecting the plant exposed to said heat shock for having an increased heat tolerance as compared to a control plant that does not comprise said nucleic acid.

2. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having 95 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:364.

3. The method of claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:364.

4. A method of increasing heat tolerance in a plant, said method comprising
introducing into a plant cell an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence comprising a polynucleotide sequence having 90 percent or greater identity to the polynucleotide sequence of SEQ ID NO:363, or wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequences having 90 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:364,
producing a plant from said plant cell,
exposing the plant produced from said plant cell to a heat shock comprising 42° C. for 5 hours,
selecting the plant exposed to said heat shock for having an increased heat tolerance as compared to a control plant that does not comprise said nucleic acid.

5. The method of claim 4 , wherein the polypeptide comprises an amino acid sequence having 95 percent or greater sequence identity to the amino acid sequence of SEQ ID NO: 364.

6. The method of claim 4 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 364.

7. The method of claim 1 , wherein said plant is a member of a species selected from the group consisting of Panicum virgatum, Sorghum bicolor, Miscanthus giganteus, Saccharum sp., Populus balsamifera, Zea mays, Glycine max, Brassica napus, Triticum aestivum, Gossypium hirsutum, Oryza sativa, Helianthus annuus, Medicago sativa, Beta vulgaris , and Pennisetum glaucum.

Assignees

• Ceres Inc

Inventors

• Christensen Cory
• Wang Wuyi
• Yang Dennis

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title