Drought and heat tolerance in plants

Patent metadata

Field	Value
Publication number	US-11578337-B2
Application number	US-202016790484-A
Country	US
Kind code	B2
Filing date	Feb 13, 2020
Priority date	Feb 15, 2008
Publication date	Feb 14, 2023
Grant date	Feb 14, 2023

Abstract

Methods and materials for modulating heat and/or drought tolerance in plants are disclosed. For example, nucleic acids encoding heat and/or drought-tolerance polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased heat and/or drought tolerance and plant products produced from plants having increased heat and/or drought tolerance.

First claim

What is claimed is:

1. A method of producing a transgenic plant, said method comprising
growing a plant transformed with an exogenous nucleic acid, wherein said exogenous nucleic acid comprising a heterologous promoter operably linked to a nucleotide sequence encoding a polypeptide comprising an amino acid sequences having 95 percent or greater amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:442; and
selecting the transgenic plant, which overexpresses said polypeptide, for exhibiting increased drought tolerance as compared to a control plant of the same species that does not comprise said exogenous nucleic acid and grown under identical growth conditions.

2. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having 97 percent or greater amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 442.

3. The method of claim 1 , wherein said polypeptide comprises the amino acid sequence as set forth in SEQ ID NO:442.

4. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having 98 percent or greater amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 442.

5. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having 99 percent or greater amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 442.

6. The method of claim 1 , wherein the nucleotide sequence comprises the polynucleotide sequence as set forth in SEQ ID NO:441.

7. The method of claim 1 , further comprising transforming a plant cell with the exogenous nucleic acid and growing the transformed plant cell comprising the exogenous nucleic acid to generate the transgenic plant comprising the exogenous nucleic acid.

8. The method of claim 1 , wherein said transgenic plant selected for exhibiting increased drought tolerance exhibits increased plant growth under limited water conditions as compared to said control plant.

9. The method of claim 8 , wherein said increased plant growth comprises increased plant height.

10. The method of claim 1 , further comprising growing the transgenic plant selected for having increased drought tolerance and producing transgenic seed from the transgenic plant.

11. The method of claim 1 , further comprising crossing the transgenic plant selected for increased drought tolerance with itself to produce transgenic seed comprising the exogenous nucleic acid and growing transgenic progeny plants from said transgenic seed.

12. The method of claim 1 , wherein selecting the transgenic plant for having increased drought tolerance comprises applying limited water conditions for at least two weeks.

13. The method of claim 1 , wherein increased drought tolerance comprises increased chronic drought tolerance.

Assignees

• Ceres Inc

Inventors

• Christensen Cory
• Wang Wuyi
• Yang Dennis

Classifications

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C07K14/415 Primary

  from plants · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title