Method of separating lipids from a lysed lipids containing biomass

Patent metadata

Field	Value
Publication number	US-11946017-B2
Application number	US-201716317305-A
Country	US
Kind code	B2
Filing date	Jul 12, 2017
Priority date	Jul 13, 2016
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

First claim

The invention claimed is:

1. A method of separating a polyunsaturated fatty acids (PUFAs)-containing lipid from the debris of a biomass, comprising the following steps:
a) providing a suspension of a biomass comprising cells which contain a PUFAs-containing lipid;
b) lysing the cells of the biomass;
c) heating the suspension obtained in step b) to a temperature of 80° C. to 100° C., and adjusting the pH to a value of 9.0 to 11.5;
d) maintaining the temperature and pH value in the ranges of paragraph c) for at least 10 hours thereby forming a demulsified suspension;
e) harvesting the PUFAs-containing oil by:
i) adding an acid to the demulsified suspension to adjust it to a pH of 5.5 to 8.5;
ii) subsequently separating the resulting oil-containing light phase from the water, salt and cell debris-containing heavy phase;

wherein:
after lysing cells in step b), and prior to steps c) and d), the suspension is concentrated to a total dry matter content of 30 to 60 wt.-% by evaporation of water;
more than 90 wt.-% of the oil contained in the initial biomass is separated and isolated;
the method is carried out without the addition of any salts for salting out the oil.

2. The method of claim 1 , wherein, in step c) the suspension of lysed cells obtained in step b) is heated to a temperature of 85° C. to 95° C., and the pH is adjusted to a value of 9.0 to 11.0 and in step d), the temperature and pH are maintained for a period of 20 to 36 hours.

3. The method of claim 1 , comprising as a further step the conversion of the water, salt, residual oil and cell debris containing heavy phase into a dried biomass with a total dry matter content of more than 90 wt.-%.

4. The method of claim 3 , wherein conversion into a dried biomass is carried out by concentrating the heavy phase to a dry matter content of 30-50 wt.-% and subsequently spray granulating using a fluidized bed granulator.

5. The method of claim 1 , wherein the suspension is provided as a fermentation broth with a biomass density of at least 80 g/l.

6. The method of claim 5 , wherein the cells are selected from algae, fungi, protists, bacteria, microalgae, plant cells, and mixtures thereof.

7. The method of claim 5 wherein the cells are of the genus Schizochytrium.

8. The method of claim 4 , wherein the suspension is provided as a fermentation broth with a biomass density of at least 80 g/l.

9. The method of claim 8 , wherein the cells are selected from algae, fungi, protists, bacteria, microalgae, plant cells, and mixtures thereof.

10. The method of claim 8 , wherein the cells are of the genus Schizochytrium.

11. The method of claim 1 , wherein separation of step e)ii) is by filtration or centrifugation.

12. The method of claim 1 , wherein, the suspension is concentrated to a total dry matter content of preferably 35 to 55 wt.-% by evaporation of water at a temperature not higher than 100° C.

13. The method of claim 12 , wherein said temperature is 80° C. to 90° C.

Assignees

• Evonik Operations Gmbh
• Dsm Ip Assets Bv

Inventors

• Bärz Manfred
• Beiser Marc
• Borchers Georg
• Cherinko Stephen Robert
• Dernedde Mathias
• Diehl Michael
• Dong Xiao Daniel
• Haberland Jürgen
• Johnson Michael Benjamin
• Kertis Robert Cody
• Lebert Jochen
• Leininger Neil Francis
• Matthews Sr Kirt Lyvell
• Pfeifer Holger
• Rabe Christian
• Resop Shannon Elizabeth Ethier
• Shank Ginger Marie
• Tarwade Vinod
• Tinsley David Allen
• Verkoeijen Daniel

Classifications

• C11B1/12 Primary

  by melting out · CPC title

• A23K10/12

  by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title

• C11B1/025 Primary

  by enzymes or microorganisms, living or dead · CPC title

• C12P7/64 Primary

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title