Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
US-10619204-B2 · Apr 14, 2020 · US
Flow cells with hydrogel coating
US11938475B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11938475-B2 |
| Application number | US-202017114134-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 7, 2020 |
| Priority date | Dec 21, 2017 |
| Publication date | Mar 26, 2024 |
| Grant date | Mar 26, 2024 |
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Abstract
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In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
First claim
Opening claim text (preview).
What is claimed is: 1. A method, comprising: applying a functionalized coating layer in depressions of a patterned flow cell substrate, wherein the depressions are separated by interstitial regions and wherein the patterned flow cell substrate includes a bonding region; grafting a primer to the functionalized coating layer to form a grafted functionalized coating layer in the depressions; applying a non-grafted hydrogel on at least the grafted functionalized coating layer, the non-grafted hydrogel being selected from the group consisting of crosslinked polyacrylamide, an agarose gel, and crosslinked polyethylene glycol; and bonding a lid to the patterned flow cell substrate at the bonding region, thereby defining a flow channel between the patterned flow cell substrate and the lid. 2. The method as defined in claim 1 , wherein the non-grafted hydrogel is disposed on the grafted functionalized coating layer and on some of the interstitial regions. 3. The method as defined in claim 1 , wherein prior to applying the functionalized coating layer, the method further comprises treating a surface of the patterned flow cell substrate to attach a functional group to the surface to form treated depressions and treated interstitial regions. 4. The method as defined in claim 3 , wherein applying the functionalized coating layer in the depressions includes: applying the functionalized coating layer in the treated depressions and on the treated interstitial regions; and polishing the functionalized coating layer from the treated interstitial regions. 5. The method as defined in claim 1 , wherein applying the non-grafted hydrogel involves applying an aqueous mixture including from about 0.001% up to about 0.1% (mass to volume) of a hydrogel material. 6. The method as defined in claim 1 , wherein the bonding region of the patterned flow cell substrate has a spacer layer bonded thereto, and wherein after the non-grafted hydrogel is applied, the bonding of the lid to the patterned flow cell substrate comprises bonding the lid to the spacer layer. 7. The method as defined in claim 1 , wherein applying the non-grafted hydrogel includes selectively depositing the non-grafted hydrogel on the grafted functionalized coating layer.
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Classifications
Agar-agar; Derivatives thereof · CPC title
characterised by the manufacture of the container or its components · CPC title
fluorescence · CPC title
Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title
Peptide nucleic acids [PNA] · CPC title
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- What does patent US11938475B2 cover?
- In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
- Who is the assignee on this patent?
- Illumina Inc, Illumina Cambridge Ltd, Ilumina Inc
- What technology area does this patent fall under?
- Primary CPC classification B01J19/0046. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Mar 26 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).