Substrates comprising nano-patterning surfaces and methods of preparing thereof

Patent metadata

Field	Value
Publication number	US-2016246170-A1
Application number	US-201415025866-A
Country	US
Kind code	A1
Filing date	Dec 17, 2014
Priority date	Dec 19, 2013
Publication date	Aug 25, 2016
Grant date	—

Abstract

Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.

First claim

1 . A substrate comprising:
a functionalizable layer comprising one or more functional groups; a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof; and a backing layer; wherein the micro-scale or nano-scale patterns of the polymer layer comprises channels, trenches, wells, posts, or combinations thereof.

2 . (canceled)

3 . The substrate of claim 1 , wherein the functionalizable layer is disposed between the backing layer and the polymer layer, and wherein at least a portion of the micro-scale or nano-scale patterns of the polymer layer perforate the polymer layer to expose the underlying functionalizable layer.

4 . (canceled)

5 . The substrate of claim 3 , wherein the functionalizable layer comprises a plurality of micro-scale or nano-scale patterns, or combinations thereof, and at least a portion of the micro-scale or nano-scale patterns of the polymer and functionalizable layers perforate the polymer and functionalizable layers to expose the underlying backing layer.

6 . The substrate of claim 1 , wherein the polymer layer is disposed between the backing layer and the functionalizable layer, and wherein the functionalizable layer comprises a plurality of micro-scale or nano-scale patterns, or combinations thereof, wherein at least a portion of the micro-scale or nano-scale patterns of the functional layer perforate the functionalizable layer to expose the underlying polymer layer.

7 . (canceled)

8 . The substrate of claim 6 , wherein at least a portion of the micro-scale or nano-scale patterns of the functionalizable and polymer layers perforate the functionalizable and polymer layers to expose the underlying backing layer.

9 . (canceled)

10 . (canceled)

11 . (canceled)

12 . The substrate of claim 1 , wherein the substrate further comprises a sealing layer to substantially seal the polymer layer and the functionalizable layer between the backing layer and said sealing layer.

13 . (canceled)

14 . The substrate of claim 1 , wherein at least one of the micro-scale or nano-scale patterns is capable of admitting a sample fluid.

15 . (canceled)

16 . The substrate of claim 1 , wherein at least a portion of the micro-scale or nano-scale patterns are posts having an average diameter of less than about 500 nm.

17 . (canceled)

18 . (canceled)

19 . (canceled)

20 . (canceled)

21 . (canceled)

22 . (canceled)

23 . (canceled)

24 . The substrate of claim 1 , wherein the functionalizable layer comprises a reactive silane layer, a functionalizable hydrogel or a functionalizable polymer.

25 . (canceled)

26 . (canceled)

27 . The substrate of claim 1 , wherein the functionalizable layer comprises a polymer or hydrogel comprising Formula (Ia) or (Ib):

R 1 is H or optionally substituted alkyl;
the functional group R A is selected from the group consisting of azide, optionally substituted amine, optionally substituted alkene, optionally substituted hydrazone, carboxylic acid, halogen, hydroxy, optionally substituted tetrazole, optionally substituted tetrazine, and thiol;
R 5 is selected from H or optionally substituted alkyl;
each of the —(CH 2 )-p can be optionally substituted;
p is an integer in the range of 1 to 50;
n is an integer in the range of 1 to 50,000; and
m is an integer in the range of 1 to 100,000.

28 . The substrate of claim 27 , wherein the functional groups comprise azides.

29 . The substrate of claim 28 , wherein the functional groups of the functionalizable layer are attached to biomolecules selected from selected from amino acids, nucleosides, nucleotides, peptides, oligonucleotides, polynucleotides, nucleic acids, proteins, or combinations thereof.

30 . (canceled)

31 . The substrate of claim 29 , wherein the biomolecules are polynucleotides or nucleic acids.

32 . The substrate of claim 1 , wherein the polymer layer comprises at least one photocurable polymer.

33 . The substrate of claim 32 , wherein the photocurable polymer comprises urethane, acrylate, silicone, epoxy, polyacrylic acid, polyacrylates, epoxysilicone, epoxy resins, polydimethylsiloxane (PDMS), silsesquioxane, acyloxysilanes, maleate polyesters, vinyl ethers, monomers with vinyl or ethynyl groups, or copolymers and combinations thereof.

34 . The substrate of claim 1 , wherein each of the backing layer, the functionalizable layer and the polymer layer is no more than about 1 mm in thickness.

35 . (canceled)

36 . (canceled)

37 . (canceled)

38 . (canceled)

39 . (canceled)

40 . The substrate of claim 1 , wherein at least one of the backing layer, polymer layer or functionalizable layer is further doped with a dopant material selected from nanoparticles, Q dots, binding receptors, ligands, nucleic acids, reactive moieties in the functionalizable layer, optical filter materials, lighter absorbing materials, light emitting materials, light scattering materials, electrical conductive materials or thermally conductive materials.

41 . (canceled)

42 . (canceled)

43 . (canceled)

44 . (canceled)

45 . (canceled)

46 . A process of preparing a substrate, comprising:
providing a substrate comprising a functionalizable layer comprising one or more functional groups disposed between a backing layer and a layer of photocurable polymer; contacting a surface of the layer of photocurable polymer with a template having a plurality of micro-scale or nano-scale patterns including micro-scale or nano-scale wells, posts, or combinations thereof; applying pressure to the template or substrate to transfer said micro-scale or nano-scale patterns to at least the layer of photocurable polymer, wherein the contacting and applying are performed at room temperature; irradiating the photocurable polymer with UV light to cure the photocurable polymer; and separating the template from substrate; wherein at least a portion of the polymer layer is perforated to expose the underlying functionalizable layer.

47 . The process of claim 46 , wherein at least a portion of polymer layer and functionalizable layer are perforated to expose the underlying backing layer.

48 .- 90 . (canceled)

91 . A substrate comprising:
a functionalizable layer comprising one or more functional groups; a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof; a backing layer; and wherein at least one of the backing layer, polymer layer or functionalizable layer is doped with a dopant material selected from nanoparticles, Q dots, binding receptors, ligands, nucleic acids, reactive moieties in the functionalizable layer, optical filter materials, lighter absorbing materials, light emitting materials, light scattering materials, electrical conductive materials or thermally conductive materials.

Assignees

• Illumina Inc

Inventors

• Bowen M Shane
• Venkatesan Bala Murali
• Barnard Steven M

Classifications

• B81B2201/05

  Microfluidics · CPC title

• B32B3/266 Primary

  characterised by an apertured layer, the apertures going through the whole thickness of the layer, e.g. expanded metal, perforated layer, slit layer regular cells B32B3/12 · CPC title

• B81B2203/0323

  Grooves · CPC title

• B32B3/30

  characterised by a layer formed with recesses or projections, e.g. {hollows, grooves, protuberances, ribs (apertured layer B32B3/266; layer with cavities or internal voids B32B3/26)} · CPC title

• B81B2203/0361

  Tips, pillars · CPC title