Cyp76ad1-beta clade polynucleotides, polypeptides, and uses thereof
US-2020354759-A1 · Nov 12, 2020 · US
CYP76AD1-beta clade polynucleotides, polypeptides, and uses thereof
US11932887B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11932887-B2 |
| Application number | US-202016940666-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 28, 2020 |
| Priority date | Sep 10, 2015 |
| Publication date | Mar 19, 2024 |
| Grant date | Mar 19, 2024 |
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Abstract
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The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as Mirabilis. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing L-DOPA from tyrosine, comprising: introducing into a cell a recombinant polynucleotide that encodes CYP76AD6, wherein said recombinant polynucleotide is under the control of a heterologous promoter active in said cell, wherein said CYP76AD6 is a tyrosine hydroxylase that converts tyrosine to L-DOPA and comprises the amino acid sequence set forth in SEQ ID NO: 18 and wherein said cell is selected from a plant cell, a bacterial cell and a yeast cell, thereby producing L-DOPA from tyrosine. 2. The method of claim 1 , wherein said method is performed without provision of L-DOPA. 3. The method of claim 1 , wherein said cell is a yeast cell. 4. The method of claim 1 , wherein said cell is a bacterial cell. 5. The method of claim 1 , further comprising introducing into the cell a nucleic acid that increases tyrosine availability in said cell. 6. The method of claim 1 , wherein said cell is a plant cell. 7. The method of claim 1 , wherein said method is performed in culture. 8. The method of claim 1 , wherein said cell is within an organism.
Assignees
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Classifications
Organic compounds containing hetero rings · CPC title
Organic compounds containing nitrogen · CPC title
Organic compounds; Microorganisms; Enzymes · CPC title
- C12P13/225Primary
Tyrosine; 3,4-Dihydroxyphenylalanine · CPC title
Human Necessities · mapped topic
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- What does patent US11932887B2 cover?
- The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a n…
- Who is the assignee on this patent?
- Yeda Res & Dev
- What technology area does this patent fall under?
- Primary CPC classification C12P13/225. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 19 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).