Genes and uses for plant enhancement

Patent metadata

Field	Value
Publication number	US-10167482-B2
Application number	US-201514757273-A
Country	US
Kind code	B2
Filing date	Dec 10, 2015
Priority date	Jun 6, 2007
Publication date	Jan 1, 2019
Grant date	Jan 1, 2019

Abstract

Transgenic seed for crops with enhanced agronomic traits are provided by trait-improving recombinant DNA in the nucleus of cells of the seed where plants grown from such transgenic seed exhibit one or more enhanced traits as compared to a control plant. Of particular interest are transgenic plants that have increased yield. The present invention also provides recombinant DNA molecules for expression of a protein, and recombinant DNA molecules for suppression of a protein.

First claim

What is claimed is:

1. A plant cell nucleus comprising a stably integrated recombinant DNA construct wherein said recombinant DNA comprises a heterologous promoter that is functional in said plant cell and that is operably linked to a protein coding DNA encoding at least one protein having an amino acid sequence comprising at least 95% amino acid sequence identity to SEQ ID NO:936 and having the function of SEQ ID NO:936
and wherein said recombinant DNA construct is stably integrated into a chromosome in a plant cell nucleus which is selected by screening a population of transgenic plants that have said recombinant DNA and an enhanced trait as compared to control plants that do not have said recombinant DNA in their nuclei; and wherein said enhanced trait is selected from group of enhanced traits consisting of increased yield and enhanced nitrogen use efficiency.

2. The plant cell nucleus comprising a recombinant DNA construct of claim 1 wherein said protein coding DNA comprises a nucleotide sequence having at least 99% nucleic acid sequence identity to SEQ ID NO:177.

3. The plant cell nucleus comprising a recombinant DNA construct of claim 1 further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type of said plant cell.

4. The plant cell nucleus of claim 3 wherein the agent of said herbicide is a glyphosate, dicamba, or glufosinate compound.

5. A transgenic plant cell or plant comprising a plurality of plant cells with the plant cell nucleus of claim 1 .

6. The transgenic plant cell or plant of claim 5 which is homozygous for said recombinant DNA.

7. A transgenic plant cell or plant comprising a plurality of plant cells with the plant cell nucleus of claim 1 .

8. The transgenic seed of claim 7 from a corn, soybean, cotton, canola, alfalfa, wheat or rice plant.

9. A transgenic pollen grain comprising a haploid gamete of a plant cell nucleus comprising a recombinant DNA construct of claim 1 .

10. A method for manufacturing non-natural, transgenic seed that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of recombinant DNA in the plant cell nucleus of claim 1 , wherein said method for manufacturing said transgenic seed comprising:
(a) screening a population of plants for said enhanced trait and said recombinant DNA, wherein individual plants in said population can exhibit said trait at a level less than, essentially the same as or greater than the level that said trait is exhibited in control plants which do not contain the recombinant DNA, wherein said enhanced trait is selected from the group of enhanced traits consisting of increased yield and enhanced nitrogen use efficiency,
(b) selecting from said population one or more plants that exhibit said trait at a level greater than the level that said trait is exhibited in control plants, and
(c) collecting seeds from selected plant selected from step b.

11. The method of claim 10 wherein said method for manufacturing said transgenic seed further comprising
(a) verifying that said recombinant DNA is stably integrated in said selected plants, and
(b) analyzing tissue of said selected plant to determine the expression or suppression of a protein having the function of a protein having SEQ ID NO:936.

12. The method of claim 10 herein said seed is corn, soybean, cotton, alafalfa, canola wheat or rice seed.

13. A method of producing hybrid corn seed comprising:
(a) acquiring hybrid corn seed from a herbicide tolerant corn plant which also has the plant cell nucleus of claim 1 ;
(b) producing corn plants from said hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA, a fraction of the plants produced from said hybrid corn seed is hemizygous for said recombinant DNA, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA,
(c) selecting corn plants which are homozygous and hemizygous for said recombinant DNA by treating with an herbicide;
(d) collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;
(e) repeating steps (c) and (d) at least once to produce an inbred corn line; and
(f) crossing said inbred corn line with a second corn line to produce hybrid seed.

Assignees

• Monsanto Technology Llc

Inventors

• Coffin Marie

Classifications

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8262

  involving plant development · CPC title

• C12N15/8251

  Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis · CPC title