Electric reaction measuring apparatus, electric reaction processing method, and recording medium

Patent metadata

Field	Value
Publication number	US-11932836-B2
Application number	US-202016920356-A
Country	US
Kind code	B2
Filing date	Jul 2, 2020
Priority date	Mar 13, 2018
Publication date	Mar 19, 2024
Grant date	Mar 19, 2024

Abstract

An electric reaction measuring apparatus measures a potential of each of measurement electrodes, which are respectively disposed in chambers in a culture vessel, with respect to at least one reference electrode. The electric reaction measuring apparatus includes at least one control circuit. A measurement object is disposed in each of the chambers. The at least one control circuit calculates at least one principal component for each of the measurement electrodes by performing principal component analysis of the potential of each of the measurement electrodes; estimates, for each of the measurement electrodes, a potential corresponding to the principal component from the principal component of the measurement electrode, and synthesizes the estimated potential; subtracts the synthesized potential of the measurement electrode from a potential measured at the measurement electrode; and outputs a potential after subtraction.

First claim

What is claimed is:

1. An apparatus comprising:
a culture vessel, wherein the culture vessel includes a partition member disposed on a bottom wall of the culture vessel, wherein the partition member includes one or more chambers, wherein one or more electrodes are disposed in the one or more chambers, wherein each of the one or more electrodes are composed of at least one reference electrode and at least one measurement electrode that are disposed about a partition wall that separates the one or more chambers from each other;
a memory storing a program; and
a processor configured to execute the program to:
measure, for each of the one or more electrodes, a potential of the at least one measurement electrode of the one or more electrodes at one or more predetermined times;
determine at least one principal component for each of the at least one measurement electrode of the one or more electrodes by performing principal component analysis of the potential of each of the at least one measurement electrode of the one or more electrodes at the one or more predetermined times;
estimate, for each of the at least one measurement electrode of the one or more electrodes, a principal component potential due to the principal component from the principal component of the at least one measurement electrode of the one or more electrodes;
synthesize the estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes;
subtract the synthesized estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes from the potential measured at the at least one measurement electrode of the one or more electrodes so as to remove noise; and
output a potential difference based on the subtraction that reflects an activity of an object in the culture vessel.

2. The apparatus according to claim 1 , further comprising:
a potential meter connected to the at least one measurement electrode of the one or more electrodes and the at least one reference electrode of the one or more electrodes.

3. The apparatus according to claim 1 , wherein the partition wall electrically separates the one or more chambers from each other.

4. The apparatus according to claim 3 ,
wherein the at least one measurement electrode of the one or more electrodes are disposed inside of the partition wall surrounding the one or more chambers, and the at least one reference electrode of the one or more electrodes are disposed on the partition wall.

5. The electric reaction measuring apparatus according to claim 1 , wherein the processor is further configured to execute the program to:
determine, for each of the at least one principal component, a contribution ratio of the at least one principal component to a variance of all measured potentials;
extract, from the at least one principal component, a plurality of the at least one principal component in descending order of the contribution ratio; and
synthesize, for each of the plurality of the at least one principal component, potentials corresponding to the plurality of the at least one principal component.

6. The apparatus according to claim 1 ,
wherein the processor is further configured to execute the program to:
determine at least two principal components for each of the at least one measurement electrode of the one or more electrodes;
determine, regarding the one or more potentials corresponding to the at least two principal components, a correlation coefficient of the one or more potentials corresponding to the at least two of the principal components;
group the two principal components whose correlation coefficient is greater than or equal to a predetermined value into one group;
determine, as a contribution ratio of the one group, a sum of a plurality of contribution ratios of all of the at least one principal component included in the one group;
extract, regarding the contribution ratio of the one group and the plurality of contribution ratios of the at least one principal component that are not included in the one group, the one group and each of the at least one principal component that are not included in the one group in descending order of the contribution ratios;
estimate a sum of potentials corresponding to the at least one principal component that are included in the extracted group and in the extracted at least one principal component that are not included in the group; and
subtract the sum of the potentials from the potential of each of the at least one measurement electrode of the one or more electrodes.

7. A method of an apparatus comprising:
acquiring, for each of one or more electrodes disposed in one or more chambers, a potential of each of at least one measurement electrode of the one or more electrodes at one or more predetermined times, wherein the apparatus comprises a culture vessel that includes a partition member disposed on a bottom wall of the culture vessel, wherein the partition member includes the one or more chambers, wherein each of the one or more electrodes are composed of at least one reference electrode and at least one measurement electrode that are disposed about a partition wall that separates the one or more chambers from each other;
determining at least one principal component for each of the at least one measurement electrode of the one or more electrodes by performing principal component analysis of the potential of each of the at least one measurement electrode of the one or more electrodes at the one or more predetermined times;
estimating, for each of the at least one measurement electrode of the one or more electrodes, a principal component potential corresponding to the principal component from the principal component of the at least one measurement electrode of the one or more electrodes;
synthesizing the estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes;
subtracting the synthesized estimated principal component potential of each of the at least one measurement electrode of the one or more electrodes from the potential acquired for the at least one measurement electrode of the one or more electrodes; and
outputting a potential difference based on the subtraction that reflects an activity of an object in the culture vessel.

8. A non-transitory computer-readable recording medium of an apparatus storing a program that causes a processor to execute a process, the process comprising:
acquiring, for each of one or more electrodes disposed in one or more chambers, a potential of each of at least one measurement electrode of the one or more electrodes at one or more predetermined times, wherein the apparatus comprises a culture vessel that includes a partition member disposed on a bottom wall of the culture vessel, wherein the partition member includes the one or more chambers, wherein one or more electrodes are disposed in the one or more chambers, wherein each of the one or more electrodes are composed of at least one reference electrode and a at least one measurement electrode that are disposed about a partition wall that separates the one or more chambers from each other;
determining at least one principal component for each of the at least one measurement of the one or more electrodes by performing principal component analysis of the potential for each of the at least one measurement of the one or more electrodes at the one or more predetermined times;
estimating, for each of the at least one measurement electrode of the one or more electrodes, a principal component potential corresponding to the principal component from the principal component of the at least one measurement electrode of the one or more electrod

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Kato Yumiko
• Tsuji Kiyotaka

Classifications

• C12M1/3407 Primary

  Measure of electrical or magnetical factor · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12M41/46 Primary

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title