Biological test system testing base

Patent metadata

Field	Value
Publication number	US-11922800-B2
Application number	US-202117244631-A
Country	US
Kind code	B2
Filing date	Apr 29, 2021
Priority date	Dec 30, 2019
Publication date	Mar 5, 2024
Grant date	Mar 5, 2024

Abstract

A test system includes a testing base including a plurality of testing base containers, and a plurality of electrodes integrated into the plurality of testing base containers. The test system further includes a plurality of drive-sense circuits coupled to the plurality of electrodes, where, when enabled, the plurality of drive-sense circuits detect changes in electrical characteristics of the plurality of electrodes. The test system further includes a processing module operably coupled to receive, from the drive-sense circuits, changes in the electrical characteristics of the plurality of electrodes, and interpret the changes in the electrical characteristics of the plurality of electrodes as impedance values representative of electrical characteristics of biological material present in the test container. The test system further includes a communication module operably coupled to communicate the electrical characteristics of the biological material.

First claim

What is claimed is:

1. A biological test system comprises:
a test container array including a plurality of test containers;
a testing base including a plurality of testing base containers, wherein the plurality of test containers fit into the plurality of test base containers;
a plurality of test container electrodes integrated into the test container array, wherein a set of test container electrodes of the plurality of test container electrodes is integrated into a test container of the test container array;
a plurality of testing base electrodes integrated into the testing base, wherein a set of testing base electrodes of the plurality of testing base electrodes is integrated into a testing base container of the plurality of testing base containers, wherein, when the test container array is inserted into the testing base, the set of test container electrodes electrically couple with the set of testing base electrodes;
a plurality of drive-sense circuits coupled to the plurality of testing base electrodes, wherein each testing base electrode of the plurality of testing base electrodes are coupled to each drive-sense circuit of the plurality of drive-sense circuits via a single line wherein, when enabled, the plurality of drive-sense circuits are operable to simultaneously drive and detect changes in electrical characteristics of the plurality of testing base electrodes via respective single lines;
a processing module coupled to the testing base, wherein the processing module is operable to:
receive a set of changes in electrical characteristics of the set of testing base electrodes; and
interpret the set of changes in electrical characteristics as a set of impedance values representative of electrical characteristics of a biological material present in the test container; and

a communication module operably coupled to the processing module, wherein the communication module is operable to communicate one or more of: the set of changes in electrical characteristics and the set of impedance values via one or more communication protocol.

2. The biological test system of claim 1 , wherein a drive-sense circuit of the plurality of drive-sense circuits is coupled to a testing base electrode of the plurality of testing base electrodes.

3. The biological test system of claim 1 further comprises:
a plurality of multiplexors coupled to the plurality of drive-sense circuits and the plurality of testing base electrodes, wherein a first multiplexor of the plurality of multiplexors is coupled to a first drive-sense circuit of the plurality of drive-sense circuits and the set of testing base electrodes; and
the processing module is further operable to:
generate a plurality of multiplexor control signals for selecting electrodes of the plurality of testing base electrodes for sensing via the plurality of drive-sense circuits, wherein a first multiplexor control signal of the plurality of multiplexor control signals selects a first testing base electrode of the set of testing base electrodes for sensing via the first drive-sense circuit.

4. The biological test system of claim 1 , wherein the biological material comprises one or more of:
one or more biological cells; and
a portion of one or more biological cells.

5. The biological test system of claim 1 , wherein the electrical characteristics of the biological material include one or more of:
position;
impedance;
size;
shape;
movement;
density;
excitability; and
potential.

6. A biological test system comprises:
a test container array including a plurality of test containers;
a testing base including a plurality of testing base containers, wherein the plurality of test containers fit into the plurality of test base containers;
a plurality of test container electrodes integrated into the test container array, wherein a set of test container electrodes of the plurality of test container electrodes is integrated into a test container of the test container array;
a plurality of testing base electrodes integrated into the testing base, wherein a set of testing base electrodes of the plurality of testing base electrodes is integrated into a testing base container of plurality of testing base containers, wherein, when the test container array is inserted into the testing base, the set of test container electrodes electrically couple with the set of testing base electrodes;
a plurality of drive-sense circuits coupled to the plurality of testing base electrodes, wherein each testing base electrode of the plurality of testing base electrodes are coupled to each drive-sense circuit of the plurality of drive-sense circuits via a single line wherein, when enabled, the plurality of drive-sense circuits are operable to simultaneously drive and detect changes in electrical characteristics of the plurality of testing base electrodes via respective single lines; and
a communication module operably coupled communicate one or more of: the set of changes in electrical characteristics and the set of impedance values via one or more of a communication protocol.

7. The biological test system of claim 6 further comprises:
a processing module operably coupled to:
receive a set of changes in electrical characteristics of the set of testing base electrodes; and
interpret the set of changes in electrical characteristics as a set of impedance values representative of electrical characteristics of a biological material present in the test container.

8. The biological test system of claim 6 , wherein a drive-sense circuit of the plurality of drive-sense circuits is coupled to a testing base electrode of the plurality of testing base electrodes.

9. The biological test system of claim 6 further comprises:
a plurality of multiplexors coupled to the plurality of drive-sense circuits and the plurality of testing base electrodes, wherein a first multiplexor of the plurality of multiplexors is coupled to a first drive-sense circuit of the plurality of drive-sense circuits and the set of testing base electrodes; and
the processing module is further operable to:
generate a plurality of multiplexor control signals for selecting electrodes of the plurality of testing base electrodes for sensing via the plurality of drive-sense circuits, wherein a first multiplexor control signal of the plurality of multiplexor control signals selects a first testing base electrode of the set of testing base electrodes for sensing via the first drive-sense circuit.

10. The biological test system of claim 6 , wherein the biological material comprises one or more of:
one or more biological cells; and
a portion of one or more biological cells.

11. The biological test system of claim 6 , wherein the electrical characteristics of the biological material include one or more of:
position;
impedance;
size;
shape;
movement;
density;
excitability; and
potential.

Assignees

• Sigmasense Llc

Inventors

• Van Ostrand Daniel Keith
• Seger Jr Richard Stuart
• Morrison Gerald Dale
• Gray Patrick Troy
• Huynh Phuong
• Markison Timothy W
• Healy Patricia M

Classifications

• G08C15/02 Primary

  simultaneously, i.e. using frequency division · CPC title

• G01D21/02

  Measuring two or more variables by means not covered by a single other subclass · CPC title

• G01N27/028 Primary

  Circuits therefor (measuring impedance per se G01R27/02) · CPC title

• G01N33/4836 Primary

  using multielectrode arrays · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title