Method of detecting or quantifying detection target in specimen, composite particle, and reagent

Patent metadata

Field	Value
Publication number	US-11899013-B2
Application number	US-202016910148-A
Country	US
Kind code	B2
Filing date	Jun 24, 2020
Priority date	Jun 25, 2019
Publication date	Feb 13, 2024
Grant date	Feb 13, 2024

Abstract

According to one embodiment, a method of detecting or quantifying a detection target in a specimen includes: irradiating a reaction mixture containing composite particles and the specimen with light to promote binding between the composite particles and the detection target; and performing measurement on the reaction mixture irradiated with the light to detect or quantify the detection target. The composite particles each include a carrier particle including two or more regions having different physical properties on a surface, and an affinity substance carried on the carrier particle and having affinity to the detection target. The light can be absorbed by at least one of the two or more regions.

First claim

The invention claimed is:

1. A method of detecting or quantifying a detection target in a specimen, the method comprising:
irradiating a reaction mixture with infrared pulse laser light, the reaction mixture containing: (1) composite particles, each comprising a carrier particle including two regions including a first region made of a metal and a second region made of a nonmetal on a surface, and an affinity substance carried on the carrier particle and having affinity to the detection target, and (2) the specimen, the infrared pulse laser light being capable of being absorbed by the first region, to promote binding between the composite particles and the detection target; and
irradiating the reaction mixture with a measurement light after the irradiation with the infrared pulse laser light, and measuring a transmitted light intensity or a scattered light intensity to detect or quantify the detection target based on a result of the measurement, wherein the irradiation with the infrared pulse laser light is stopped during the irradiation with the measurement light.

2. The method according to claim 1 , wherein the metal is gold, silver, copper, or iron.

3. The method according to claim 1 , wherein the nonmetal is an inorganic substance or a polymer.

4. The method according to claim 1 , wherein the affinity substance is an antigen or an antibody.

5. The method according to claim 1 , wherein the affinity substance is selectively carried on the second region of the carrier particle.

6. The method according to claim 1 , wherein the first region has a surface area of 0.35 to 0.45, when the entire surface area of the carrier particle is defined as 1.

7. The method according to claim 1 , wherein the measurement light has a wavelength within a range of 340 to 800 nm.

8. The method according to claim 1 , wherein the carrier particle has an average particle diameter of 20 to 800 nm.

Assignees

• Canon Medical Systems Corp

Inventors

• Sugita Satoru
• Tahara Hirotoshi

Classifications

• G01N33/551 Primary

  the carrier being inorganic · CPC title

• G01N21/17

  Systems in which incident light is modified in accordance with the properties of the material investigated (where the material investigated is optically excited causing a change in wavelength of the incident light G01N21/63) · CPC title

• G01N21/75

  Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated (systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73) · CPC title

• G01N25/18

  by investigating thermal conductivity (by calorimetry G01N25/20; by measuring change of resistance of an electrically-heated body G01N27/18) · CPC title

• G01N25/482

  concerning the temperature responsive elements (measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00) · CPC title