Systems including janus droplets capable of binding an analyte and changing orientation to provide a detectable change

Patent metadata

Field	Value
Publication number	US-10060913-B2
Application number	US-201615269543-A
Country	US
Kind code	B2
Filing date	Sep 19, 2016
Priority date	Sep 19, 2016
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

Embodiments described herein may be useful in the detection of analytes. The systems and methods may allow for a relatively simple and rapid way for detecting analytes such as chemical and/or biological analytes and may be useful in numerous applications including sensing, food manufacturing, medical diagnostics, performance materials, dynamic lenses, water monitoring, environmental monitoring, detection of proteins, detection of DNA, among other applications. For example, the systems and methods described herein may be used for determining the presence of a contaminant such as bacteria (e.g., detecting pathogenic bacteria in food and water samples which helps to prevent widespread infection, illness, and even death). Advantageously, the systems and methods described herein may not have the drawbacks in current detection technologies including, for example, relatively high costs, long enrichment steps and analysis times, and/or the need for extensive user training. Another advantageous feature provided by the systems and methods described herein includes fabrication in a relatively large scale. In some embodiments, the systems and methods may be used in conjunction with a detector including handheld detectors incorporated with, for example, smartphones (e.g., for the on-site detection of analytes such as pathogenic bacteria).

First claim

What is claimed is:

1. A system comprising:
a plurality of Janus droplets associated with binding moieties to an analyte, the plurality of Janus droplets comprising an interface between a first phase and a second phase of the droplets, wherein the binding moiety and analyte are selected such that when the analyte binds to the binding moiety at least a portion of the plurality of Janus droplets are changed from a first orientation, to a second orientation different than the first orientation, sufficient to change electromagnetic radiation interacting with the plurality of Janus droplets in a detectable manner, and wherein the plurality of Janus droplets are optically transparent in a direction perpendicular to a surface of the interface in the first orientation.

2. A system as in claim 1 , wherein upon binding to the binding moieties, at least a portion of the plurality of Janus droplets agglutinate.

3. A system as in claim 1 , wherein, prior to binding to the binding moieties, the plurality of Janus droplets are oriented such that at least a portion of interfaces between the first phase and the second phase within each Janus droplet are aligned parallel with respect to one another.

4. A system as in claim 1 , wherein, prior to the analyte binding to the binding moieties, the plurality of Janus droplets are bound to a surface.

5. A method for changing the optical transmission of an article, comprising:
exposing, to an article comprising a surface, an outer phase adjacent the surface, and a plurality of Janus droplets dispersed within the outer phase, a chemical or biological analyte, wherein the chemical or biological analyte, if present, interacts with at least a portion of the article such that at least a portion of the plurality of Janus droplets change orientation thereby changing the optical transmission of the article, wherein the plurality of Janus droplets have at least one orientation that is optically transparent in at least one direction perpendicular to the surface.

6. A method as in claim 5 , wherein the plurality of Janus droplets comprises one or more amphiphilic compounds including at least one binding moiety.

7. A method as in claim 6 , wherein at least a portion of the plurality of Janus droplets are bound to a surface of the article via the binding moiety.

8. A method as in claim 7 , wherein, upon exposing the article to a chemical or biological analyte, at least a portion of the plurality of Janus droplets unbind from the surface.

9. A method as in claim 6 , wherein interacting with at least a portion of the article comprises binding of the chemical or biological analyte to the at least one binding moiety.

10. A method as in claim 5 , wherein, prior to exposing the article to a chemical or biological analyte, at least a portion of the plurality of Janus droplets are oriented such that at least a portion of interfaces between a first phase and a second phase within each Janus droplet are aligned parallel with respect to one another.

11. A method as in claim 10 , wherein substantially all of the interfaces between a first phase and a second phase within each Janus droplet are aligned parallel with respect to one another.

12. A method as in claim 5 , wherein, upon exposing the article to a chemical or biological analyte, at least a portion of the plurality of Janus droplets agglutinate.

13. A method as in claim 5 , wherein, upon exposing the article to a chemical or biological analyte, at least a portion of the plurality of Janus droplets are oriented such that at least a portion of interfaces between a first phase and a second phase within each Janus droplet are not aligned parallel with respect to one another.

14. An article, comprising:
a surface;
an outer phase deposited on at least a portion of the surface; and
a plurality of Janus droplets dispersed within the outer phase,
wherein the plurality of Janus droplets are optically transparent in at least one orientation in a direction perpendicular to the surface,
wherein at least a portion of the plurality of Janus droplets comprise an amphiphilic compound including at least one binding moiety, and
wherein at least a portion of the plurality of Janus droplets are bound to the surface via the binding moiety.

15. An article as in claim 14 , wherein at least a portion of the plurality of Janus droplets are oriented such that an interface between a first phase and a second phase within each Janus droplet are not aligned parallel to the surface.

16. An article as in claim 14 , wherein, upon exposure of the plurality of Janus droplets to a biological or chemical analyte, at least a portion of Janus droplets unbind from the surface.

17. An article as in claim 16 , wherein the outer phase is substantively optically transmissive relative to a direction perpendicular to the surface after exposure of the plurality of Janus droplets to the biological or chemical analyte.

18. An article as in claim 14 , wherein, upon exposure of the plurality of Janus droplets to a biological or chemical analyte, at least a portion of Janus droplets change orientation.

19. An article as in claim 14 , wherein, upon exposure of the plurality of Janus droplets to a chemical or biological analyte, the plurality of Janus droplets increase in optical transmission relative to a direction perpendicular to the surface.

20. An article as in claim 14 , wherein each Janus droplet comprises a first phase and a second phase, immiscible with the first phase.

21. An article as in claim 14 , wherein the outer phase is an aqueous phase.

22. An article as in claim 14 , wherein the first phase comprises a hydrocarbon, a fluorocarbon, a silicone, a liquid crystal, an ionic liquid, a polymer, combinations thereof, or derivatives thereof.

23. An article as in claim 14 , wherein the second phase comprises a hydrocarbon, a fluorocarbon, a silicone, a liquid crystal, an ionic liquid, a polymer, combinations thereof, or derivatives thereof, immiscible with the first phase.

24. An article as in claim 14 , wherein the amphiphilic compound is selected from the group consisting of: ionic surfactants, non-ionic surfactants, zwitterionic surfactants, polymers, proteins, DNA, RNA, acids, carbohydrates, saccharides, enzymes, chromophores, lipids, graphene oxide, combinations thereof, and derivatives thereof.

25. An article as in claim 14 , wherein an interface between the outer phase and the plurality of Janus droplets comprises the amphiphilic compound.

26. An article as in claim 14 , wherein the analyte comprises a biological compound, a drug, a macromolecule, a salt, an electrolyte, an enzyme, an acid, a nucleic acid, a carbohydrate, a peptide, a protein, a phosphate, a sulfonate, a virus, a pathogen, an oxidant, a reductant, a toxin, a chemical warfare agent, an explosive, carbon dioxide, or combinations thereof.

27. A system, comprising:
an article, comprising:
a surface,
an outer phase deposited on at least a portion of the surface, and
a plurality of Janus droplets dispersed within the outer phase,
wherein the plurality of Janus droplets are optically transparent in at least one orientation in a direction perpendicular to the surface,
wherein at least a portion of the plurality of Janus droplets comprise an amphiphilic compound including at least one binding moiety, and
wherein at least a portion of the plurality of Janus droplets are bound to the surface via t

Assignees

• Massachusetts Inst Technology

Inventors

• Swager Timothy M
• Zhang Qifan
• Savagatrup Suchol

Classifications

• G01N33/5432 Primary

  Liposomes or microcapsules · CPC title

• C12Q1/6834

  Enzymatic or biochemical coupling of nucleic acids to a solid phase · CPC title

• G01N2021/7783

  Transmission, loss · CPC title

• G01N21/59

  Transmissivity (G01N21/25 takes precedence) · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title